IL33 Antibody (OAPB00547)

Catalog No: OAPB00547

Size:100UG

Western blot analysis of IL-33 in human lymph node tissue lysate with IL-33 antibody at (A) 1 and (B) 2 ug/mL.

Immunohistochemistry of IL-33 in human lymph node tissue with IL-33 antibody at 2.5 ug/ml.

Immunofluorescence of IL-33 in Human Lymph Node tissue with IL-33 antibody at 20 ug/mL.

Product Info

• Predicted Species Reactivity: Human|Mouse|Rat
• Product Format: Liquid. IL-33 Antibody is supplied in PBS containing 0.02% sodium azide.
• Clonality: Polyclonal
• Isotype: IgG
• Host: Rabbit
• Conjugation: Unconjugated
• Application: Enzyme-linked immunosorbent assay|Immunofluorescence|Immunohistochemistry-Paraffin|Western blot
• Reconstitution and Storage: IL-33 antibody can be stored at 4C for three months and -20C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
• Immunogen: Anti-IL-33 antibody (4273) was raised against a 17 amino acid synthetic peptide from near the center of human IL-33. The immunogen is located within the 150-200 amino acids of IL-33.
• Purification: Affinity Purified
• Predicted Homology Based on Immunogen Sequence: Human; Mouse; Rat
• Concentration: 1 mg/ml
• Blocking Peptide: For anti-IL33 (OAPB00547) antibody is Catalog # OEPB02664
• Application Info: IL-33 antibody can be used for the detection of IL-33 by Western blot at 1 - 2 ug/mL. Observed: 35 kDa
  For immunofluorescence start at 20 g/mL.
  Antibody can also be used for immunohistochemistry starting at 2.5 g/mL.
• Reference: Schmitz J, Owyang A, Oldham E, et al. IL-33, and interleukin-1-like cytokine that signals via the IL-1 receptor-related protein ST2 and induces T helper type 2-associated cytokines. Immunity2005; 23:479-90.;
  Dinarello CA. Interleukin-18, a proinflammatory cytokine. Eur. Cytokine Netw.2000; 11:483-6.;
  Brint EK, Xu D, Liu H, et al. ST2 is an inhibitor of interleukin 1 receptor and Toll-like receptor 4 signaling and maintains endotoxin tolerance. Nat. Immunol.2004; 5:373-9.;
  Chackerian AA, Oldham ER, Murphy EE, et al. IL-1 receptor accessory protein and ST2 comprise the IL-33 receptor complex. J. Immunol.2007; 179:2551-5.

Target Info

• Gene Symbol: IL33
• Gene Full Name: interleukin 33
• Alias Symbols: C9orf26;DVS27;DVS27-related protein;IL1F11;interleukin-1 family member 11;interleukin-33;NFEHEV;NF-HEV;nuclear factor for high endothelial venules;nuclear factor from high endothelial venules.
• NCBI Gene Id: 90865
• Protein Name: Interleukin-33|interleukin-33 isoform a precursor [Homo sapiens]
• Description of Target: Cytokine that binds to and signals through the IL1RL1/ST2 receptor which in turn activates NF-kappa-B and MAPK signaling pathways in target cells (PubMed:16286016). Involved in the maturation of Th2 cells inducing the secretion of T-helper type 2-associated cytokines. Also involved in activation of mast cells, basophils, eosinophils and natural killer cells. Acts as a chemoattractant for Th2 cells, and may function as an "alarmin", that amplifies immune responses during tissue injury (PubMed:17853410, PubMed:18836528).
• Uniprot ID: O95760
• Protein Accession #: https://www.ncbi.nlm.nih.gov/protein/NP_254274
• Nucleotide Accession #: NM_001199640.1; NM_001199641.1; NM_033439.3; XM_005251626.3; XM_005251627.1
• Molecular Weight: Predicted: 31kD Observed: 33kD