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Catalog No: OKAA00021-96W
Size:96 Wells
Price: $450.00
Availability: Domestic: within 1-2 weeks delivery | International: 1-2 weeks
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Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Product Info
Predicted Species ReactivityHuman
ELISA Kit Detection MethodColorimetric, OD450 nm
ELISA Kit ReproducibilityThe coefficient of variation of both intra-assay and inter-assay were less than 10%.
ELISA Kit Component
2 vials human IL-32alpha Standard lyophilized, 500 pg/ml upon reconstitution
2 vials concentrated Biotin-Conjugate anti-human IL-32alpha monoclonal antibody
2 vials Streptavidin-HRP solution,
1 bottle Standard /sample Diluent
1 bottle Biotin-Conjugate antibody Diluent
1 bottle Streptavidin-HRP Diluent
1 bottle Wash Buffer Concentrate 20x (PBS with 1% Tween-20)
1 vial Substrate Solution
1 vial Stop Solution
2 vials human Leptin Standard lyophilized, 2000 pg/ml upon reconstitution
2 vials concentrated Biotin-Conjugate anti-human Leptin antibody
Additional InformationSample Type: cell culture supernates, serum, and plasma
::Reagents: REAGENTS
1. Aluminum pouches with a Microwell Plate coated with monoclonal antibody to human IL-32?(8x12)
2. 2 vials human IL-32? Standard lyophilized, 500 pg/ml upon reconstitution
3. 2 vials concentrated Biotin-Conjugate anti-human IL-32? monoclonal antibody
4. 2 vials Streptavidin-HRP solution,
5. 1 bottle Standard /sample Diluent
6. 1 bottle Biotin-Conjugate antibody Diluent
7. 1 bottle Streptavidin-HRP Diluent
8. 1 bottle Wash Buffer Concentrate 20x (PBS with 1% Tween-20)
9. 1 vial Substrate Solution
10. 1 vial Stop Solution
11. 3 pieces Adhesive Films
12. package insert
Sample Typecell culture, supernates, serum, and plasma
Sensitivity7 pg/ml
Predicted Homology Based on Immunogen SequenceHuman
SpecificityThis assay recognizes both natural and recombinant human IL-22. The factors listed below were prepared at 50ng/ml in Standard /sample Diluent and assayed for cross-reactivity and no significant cross-reactivity or interference was observed.
Recombinant human: IL-1a, IL-1b, IL-2, IL-3, IL-4, IL-5, IL-6 ,
Recombinant mouse: IL-1a, IL-1b, IL-2, IL-5, IL-6, IL-7 ,
Recombinant rat: IL-2, IL-4, IL-6, IL-10, IL-22
Assay InfoAssay Method: Quantitative Sandwich ELISA
Gene SymbolIL32
Gene Full Nameinterleukin 32
Alias SymbolsNK4, TAIF, TAIFa, TAIFb, TAIFc, TAIFd, IL-32beta, IL-32alpha, IL-32delta, IL-32gamma
NCBI Gene Id9235
Protein NameInterleukin-32
Description of TargetThis gene encodes a member of the cytokine family. The protein contains a tyrosine sulfation site, 3 potential N-myristoylation sites, multiple putative phosphorylation sites, and an RGD cell-attachment sequence. Expression of this protein is increased after the activation of T-cells by mitogens or the activation of NK cells by IL-2. This protein induces the production of TNFalpha from macrophage cells. Alternate transcriptional splice variants, encoding different isoforms, have been characterized.
Uniprot IDP24001
Protein Accession #NP_001012649.1
Nucleotide Accession #NM_001012631.1
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