IL2RA Antibody : PE (OATA00320)

Catalog No: OATA00320

Human peripheral blood lymphocytes were stained with 5 uL (0.06 ug) PE Anti-Human CD25 (OATA00320) (right panel) or 0.06 ug PE Mouse IgG1 isotype control (left panel).

Product Info

• Tested Species Reactivity: Human
• Predicted Species Reactivity: Baboon, Chimpanzee, Cynomolgus, Rhesus
• Product Format: Liquid. 10 mM NaH2PO4, 150 mM NaCl, 0.09% NaN3, 0.1% gelatin, pH7.2
• Clonality: Monoclonal
• Clone: BC96
• Isotype: Mouse IgG1, kappa
• Host: Mouse
• Conjugation: PE: Phycoerythrin
• Application: FC
  Excitation Laser: Blue (488 nm), Green (532 nm), Yellow-Green (561 nm)
  Emmision: 578 nm
• Reconstitution and Storage: 2-8C protected from light
• Specificity: CD25
• Application Info: This antibody preparation has been pre-titrated and quality-tested for flow cytometry using an appropriate cell type. The antibody has been diluted for use at 5 uL per test, defined as the amount of antibody that will stain a cell sample in a final volume of approximately 100 uL. The number of cells within a sample should be determined empirically, but typically ranges between 1x10e5 to 1x10e8 cells.
• Reference:

  Willer DO, Ambagala APN, Pilon R, chan JK, Fournier J, Brooks J, Sandstrom P, and MacDonald KS. 2012. J. Virol. 86: 3626-3634. (Flow cytometry - cynomolgus)

  Ruffell B, Au A, Rugo HS, Esserman LJ, Hwang ES, and Coussens LM. 2012. Proc. Natl. Acad. Sci. 109: 2796-2801. (Flow cytometry)

  Mackroth MS, Malhotra I, Mungai P, Koech D, Muchiri E, and King CL. 2011. J. Immunol. 186: 2780-2791. (Flow cytometry)

  Mori T, Miyamoto T, Yoshida H, Asakawa M, Kawasumi M, Kobayashi T, Morioka H, Chiba K, Toyama Y, and Yoshimura A. 2011. Int. Immunol. 23(11):701-712. (Cell sorting / negative selection - T cells)

  Wan Q, Kozhaya L, ElHed A, Ramesh R, Carlson TJ, Djretic IM, Sundrud MS, and Unutmax D. 2011. J. Exp. Med. 208: 1875-1887. (Cell sorting / negative selection - T cells)

  Verhoeven D, Sankaran S, Silvey M, and Dandekar S. 2008. J. Virol. 82: 4016-4027. (Flow cytometry - rhesus macaque)

Target Info

• Gene Symbol: IL2RA
• Gene Full Name: interleukin 2 receptor subunit alpha
• Alias Symbols: p55, CD25, IL2R, IMD41, TCGFR, IDDM10
• NCBI Gene Id: 3559
• Protein Name: interleukin-2 receptor subunit alpha
• Description of Target: The interleukin 2 (IL2) receptor alpha (IL2RA) and beta (IL2RB) chains, together with the common gamma chain (IL2RG), constitute the high-affinity IL2 receptor. Homodimeric alpha chains (IL2RA) result in low-affinity receptor, while homodimeric beta (IL2RB) chains produce a medium-affinity receptor. Normally an integral-membrane protein, soluble IL2RA has been isolated and determined to result from extracellular proteolyisis. Alternately-spliced IL2RA mRNAs have been isolated, but the significance of each is presently unknown. Mutations in this gene are associated with interleukin 2 receptor alpha deficiency.
• Uniprot ID: P01589
• Protein Accession #: NP_000408.1
• Nucleotide Accession #: NM_000417.2