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Il2ra Antibody (OASA05092)

0.25 mg
$459.00
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Gene Symbol:
Il2ra
NCBI Gene Id:
16184
Protein Name:
Interleukin-2 receptor subunit alpha
Swissprot Id:
P01590
Protein Accession #:
NP_032393.3
Alias Symbols:
CD25, Il2r, Ly-43, MGC130561, Il2ra
Replacement Item:
This antibody may replace item sc-13946 from Santa Cruz Biotechnology.
Description of Target:
RAT ANTI MOUSE CD25
Protein Size (# AA):
268
Host:
Rat
Clonality:
Monoclonal
Application:
IHC-AFF, FC, IP
Tissue Tool:
Find tissues and cell lines supported by DNA array analysis to express Il2ra.
RNA Seq:
Find tissues and cell lines supported by RNA-seq analysis to express Il2ra.
Immunogen:
The immunogen for anti-Il2ra antibody: b6.1 CTL cell line
Predicted Species Reactivity:
Mouse
Predicted Homology Based on Immunogen Sequence:
Mouse
Product Format:
Purified IgG - liquid
Reconstitution and Storage:
Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Datasheets/Manuals:
Printable datasheet for anti-Il2ra antibody - OASA05092
Specificity:
CD25
Clone:
PC61.5.3
Isotype:
IgG1
Application Info:
Flow Cytometry: Use 10ul of the suggested working dilution to label 1 x 106 cells in 100ul.
Additional Information:
Fusion Partners: Spleen cells from immunised OFA rats were fused with cells of the P3X63Ag8.653 mouse myeloma cell line.
:::
Preparation: Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Preservative Stabilisers: 0.09% - Sodium Azide (NaN3)
:::
Approx Protein Conc: IgG concentration 1.0 mg/ml
Target Reference:
1. Moreau, J-L. et al. (1987) Monoclonal Antibodies identify three epitope clusters on the mouse p55 subunit of the interleukin 2 receptor: relationship to the interleukin 2 binding site. Eur. J. Immunol. 17:1835 - 1838
2. Hashimoto, N. et al. (1986) Dissociation of interleukin 2 dependent and independent B cell proliferation with monoclonal anti-interleukin 2 receptor antibody. Eur. J. Immunol. 16: 317-320.
3. Lowenthal, J.W. et al. (1985) High and low affinity IL-2 receptors: Analysis by IL-2 dissociation rate and reactivity with monoclonal anti-receptor antibody PC61. J. Immunol. 135: 3988-3994.
4. Ceredig, R. et al. (1985) Expression of interleukin-2 receptor as a differentiation marker on intrathymic stem cells. Nature 314: 98-100.
5. Yaqoob, P. and Calder, P.C. (1997) Glutamine Requirement of Proliferating T lymphocytes. Nutrition 13: 646-651.

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