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Il2ra Antibody (OASA03915)

Catalog#: OASA03915
This product is Genway GWB-891A5C
More Information
Predicted Species Reactivity Rat
Product Format Purified IgG - liquid
Clonality Monoclonal
Clone OX-39
Isotype IgG1
Host Mouse
Application IHC-AFF, FC, IP, IHC-FFPE
Additional Information Fusion Partners: Spleen cells from immunised BALB/c mice were fused with cells of the NSO/1 mouse myeloma cell line.
:: Preparation: Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Preservative Stabilisers: 0.09% - Sodium Azide
:: Approx Protein Conc: IgG concentration 1.0 mg/ml
Buffer Solutions: Phosphate buffered saline pH7.4
Reconstitution and Storage Store at +4oC or at -20oC if preferred.
This product should be stored undiluted.
Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Replacement Item This antibody may replace item sc-13946 from Santa Cruz Biotechnology.
Immunogen The immunogen for anti-Il2ra antibody: stimulated Rat T cells
Predicted Homology Based on Immunogen Sequence Rat
Datasheets/Manuals Printable datasheet for anti-Il2ra antibody - OASA03915
Specificity CD25
Application Info PLP fixation is recommended, see Whiteland. J.L. et al.(1995)for details.
Application Data Application #1: Staining of ConA activated rat splenic lymphocytes with Mouse anti Rat CD25:RPE
Application #2: Staining of Con A activated rat spleen cells with Mouse anti Rat CD25: Biotin
Application #3: Staining of rat spleen cells with Mouse anti Rat CD25: FITC
Application #4: Staining of stimulated rat spleen cells with Mouse anti Rat CD25:FITC
Protocol Information Citation: 1: López-Guerrero JA, López-Bote JP, Ortiz MA, Gupta RS, Páez E, Bernabeu C. Modulation of adjuvant arthritis in Lewis rats by recombinant vaccinia virus expressing the human 60-kilodalton heat shock protein. Infect Immun. 1993 Oct;61(10):4225-31. PubMed PMID: 8406810; PubMed Central PMCID: PMC281148.
Species: Rat
Experiment Name: Flow cytometric analysis
Experiment Background: 1. JOSE et al. performed flow cytometry to analysis of lymph node cells from CFA-treated rats infected with hsp60-VV. 2. Popliteal lymph node cells were obtained and incubated for 2 days in the presence of fhsp60 or concanavalin A.
Experimental Steps: 1. Popliteal lymph node cells of Lewis rats to be stained for flow cytometric analysis were obtained.2. Lymphocytes were separated on a Ficollmetrizoate (Pharmacia) gradient, washed three times with 0.1% bovine serum albumin (BSA) in PBS containing 10% normal rat serum, and incubated for 30 min at 4C with the appropriate monoclonal antibodies in PBS-BSA.3. Anti-CD2 (MRC OX-34), anti-CD4 (W3-25), anti-CD8 (MRC OX-8), and anti-interleukin-2-receptor (MRC OX-39) antibodies were used to stain (Bicester, United Kingdom) and cells were then washed.4. They were then incubated with rabbit anti-mouse immunoglobulin G (IgG) [F(ab')2 fragment]conjugated to fluorescein in PBS-BSA containing 10% normal rat serum.5. Cells were finally washed three times and analyzed in an EPICS-C flow cytofluorometer (Coulter Scientific, M6stoles, Spain).
Other Reagents Used: 2-mercaptoethanol,glutamine
Number Of Protocols: 1
Target Reference 1. Paterson, D.J. et al. (1987) Antigens of Activated Rat T Lymphocytes including a molecule of 50,000 Mr, detected only on CD4 positive T Blasts. Mol. Immunol. 24: 1281-1290.
2. Charteris, D.G. and Lightman, S.L. (1993) In vivo lymphokine production in experimental autoimmune uveoretinitis Immunology. 78 : 387 - 392
3. Hayosh, N.S. and Swanborg, R.H. (1987) Autoimmune effector cells. IX. Inhibition of adoptive transfer of autoimmune encephalomyelitis with a monoclonal antibody specific for interleukin 2 receptors J. Immunol. 138: 3771-3775.
4. Tellides, G. et al. (1987) Functional blocking of the Interleukin-2 receptor (IL-2R), may be important in the efficacy of IL-2R antibody therapy. Transplant. Proc. XIX: 4231-4233.
5. Signore, A. et al. (1987) Detection of activated lymphocytes in endocrine pancreas of BB/W rats by injection of 123I-interleukin-2: an early sign of type 1 diabetes Lancet 2 (8558): 537-40.
6. Whiteland, J.L. et al. (1995) Immunohistochemical detection of T-cell subsets and other leukocytes in paraffin-embedded rat and mouse tissues with monoclonal antibodies. J. Histochem. Cytochem. 43: 313-320.
Gene Symbol Il2ra
Alias Symbols IL2RAC, Il2ra
NCBI Gene Id 25704
Protein Name Interleukin-2 receptor subunit alpha
Description of Target MOUSE ANTI RAT CD25
Swissprot Id P26897
Protein Accession # NP_037295.1
Protein Size (# AA) 267
Tissue Tool Find tissues and cell lines supported by DNA array analysis to express Il2ra.
RNA Seq Find tissues and cell lines supported by RNA-seq analysis to express Il2ra.
  1. What is the species homology for "Il2ra Antibody (OASA03915)"?

    The tested species reactivity for this item is "". This antibody is predicted to have homology to "Rat".

  2. How long will it take to receive "Il2ra Antibody (OASA03915)"?

    This item is available "Domestic: within 1 week delivery | International: 1 week".

  3. What buffer format is "Il2ra Antibody (OASA03915)" provided in?

    This item is provided in "Purified IgG - liquid ".
    Additional format options may be available. For more information please contact

  4. What are other names for "Il2ra Antibody (OASA03915)"?

    This target may also be called "IL2RAC, Il2ra" in publications.

  5. What is the shipping cost for "Il2ra Antibody (OASA03915)"?

    The shipping cost for this item is $40 within the US. Please contact us for specific shipping prices for international orders.

  6. What is the guarantee for "Il2ra Antibody (OASA03915)"?

    All Aviva products have been through rigorous validations and carry 100% satisfaction guarantee.

  7. Can I get bulk pricing for "Il2ra Antibody (OASA03915)"?

    You can get bulk pricing for this item by going here.

  8. What is the molecular weight of the protein?

    The molecular weight reported by Uniprot for this item is "".
    Please note observed molecular weights in western blot applications may differ depending on a variety of protein characteristics.

  9. What protocols are available for "Il2ra Antibody (OASA03915)"?

    We may have detailed protocol data avaialble for this item. To learn more, please view the "Protocols & Data tab on the product page.

  10. What are positive controls for "IL2RA"?

    We have listed RNA Seq and gene expression data in the "Target Info" tab. You may be able to find adequate positive controls there.

  11. What are negative controls for "IL2RA"?

    We have listed RNA Seq and gene expression data in the "Target Info" tab. You may be able to find adequate positive controls there.

  12. What other proteins interact with "IL2RA"?

    This protein has been reported to interact with "Protein Interactions". Please view the "Related Categories" tab on the product page for more information.

  13. What biological processes are associated with "IL2RA"?

    This protein has been associated with "Biological Processes". Please view the "Related Categories" tab on the product page for more information.

  14. What cellular components are associated with "IL2RA"?

    This protein has been associated with "Cellular Components". Please view the "Related Categories" tab on the product page for more information.

  15. What protein functions are associated with "IL2RA"?

    This protein has been associated with "Protein Functions". Please view the "Related Categories" tab on the product page for more information.

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