Il25 ELISA Kit (Rat) (OKCD01599)

Catalog No: OKCD01599 (Formerly GWB-KBBNX1)

Size:96WELLS

Rat Il25 ELISA Kit Standard Curve

Product Info

• Predicted Species Reactivity: Rat|Rattus norvegicus
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric
• ELISA Kit Duration: 3h
• ELISA Kit Linearity:

  Sample	1:2 Dilution	1:4 Dilution	1:8 Dilution	1:16 Dilution
  Serum (n=5)	86-95%	80-96%	78-101%	89-103%
  EDTA Plasma (n=5)	97-104%	78-93%	87-94%	88-102%
  Heparin Plasma (n=5)	95-104%	88-96%	95-105%	85-99%

• ELISA Kit Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Interleukin 25 (IL25). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Interleukin 25 (IL25). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Interleukin 25 (IL25), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Interleukin 25 (IL25) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
• ELISA Kit Range: 7.8-500pg/mL
• ELISA Kit Recovery:

  Matrix	Recovery Range (%)	Average (%)
  Serum (n=5)	95-102	99
  EDTA Plasma (n=5)	85-92	88
  Heparin Plasma (n=5)	95-102	98

• ELISA Kit Reproducibility: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Interleukin 25 (IL25) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Interleukin 25 (IL25) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
• ELISA Kit Component:
  • Anti-Il25 Microplate: 96 Wells (12 x 8 Well Strips)
  • Il25 Lyophilized Standard: 2 x 500 pg
  • 100X Biotinylated Il25 Detector Antibody: 120 uL
  • Avidin/HRP Conjugate: 120 uL
  • Standard Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 30X Wash Buffer: 1 x 20 mL
  • TMB Substrate: 1 x 9 mL
  • Stop Solution: 1 x 6 mL
• Reconstitution and Storage: 2°C to 8°C|-20°C
• Sample Type: Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
• Sensitivity: < 2.8 pg/mL
• Specificity: This assay has high sensitivity and excellent specificity for detection of Interleukin 25 (IL25). No significant cross-reactivity or interference between Interleukin 25 (IL25) and analogues was observed.
• Assay Info: Assay Methodology: Quantitative Sandwich Immunoassay

Target Info

• Gene Symbol: Il25
• Gene Full Name: interleukin 25
• Alias Symbols: interleukin-25;RGD1561632.
• NCBI Gene Id: 501996
• Protein Name: Interleukin 25
• Description of Target: Interleukin 25
• Uniprot ID: D3ZLB1
• Protein Accession #: NP_001178936.1
• Nucleotide Accession #: NM_001192007.1
• Protein Size (# AA): 169