IL25 Antibody - middle region (OAAB10988)

Catalog No: OAAB10988

Size:400ul

IL25 Antibody (Center) (Cat. #OAAB10988) western blot analysis in ZR-75-1 cell line lysates (35ug/lane).This demonstrates the IL25 antibody detected the IL25 protein (arrow).

IL25 is strongly supported by BioGPS gene expression data to be expressed in ZR75-1

All lanes : Anti-IL25 Antibody (Center) at 1:1000 dilution Lane 1: human testis lysate Lane 2: PC-3 whole cell lysate Lysates/proteins at 20 ug per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 20 kDa Blocking/Dilution buffer: 5% NFDM/TBST.

IL25 Antibody (Center) (OAAB10988)immunohistochemistry analysis in formalin fixed and paraffin embedded human testis tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of IL25 Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.

Product Info

• Predicted Species Reactivity: Human
• Product Format: Liquid. Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide.
• Clonality: Polyclonal
• Isotype: Ig
• Host: Rabbit
• Application: WB, IHC-P
• Additional Information: Function: Induces activation of NF-kappa-B and stimulates production of the proinflammatory chemokine IL-8. Proinflammatory cytokine favoring Th2-type immune responses.
  Cellular Location: Secreted.
  Tissue Location: Expressed at low levels in several tissues, including brain, kidney, lung, prostate, testis, spinal cord, adrenal gland, and trachea.
• Reconstitution and Storage: Maintain refrigerated at 2-8C for up to 2 weeks. For long term storage store at -20C in small aliquots to prevent freeze-thaw cycles.
• Immunogen: This IL25 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 76-104 amino acids from the Central region of human IL25.
• Purification: This antibody is purified through a protein A column, followed by peptide affinity purification.
• Predicted Homology Based on Immunogen Sequence: Human
• Concentration: Approximately 0.5mg/ml. Actual concentration varies with each lot.
• Sample Type Confirmation:

  IL25 is strongly supported by BioGPS gene expression data to be expressed in ZR75-1

• Intended Use: IL25 Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures.
• Application Info: WB: 1:1000
  IHC-P: 1:10~50
• Reference: Xu, G., et al. Allergy 65(5):581-589(2010)
  Davila, S., et al. Genes Immun. 11(3):232-238(2010)
  Goswami, S., et al. Nat. Immunol. 10(5):496-503(2009)
  Caruso, R., et al. Blood 113(15):3512-3519(2009)
  Garley, M., et al. Adv Med Sci 53(2):326-330(2008)
• Publications:

  Expression of IL-25, IL-33, and Thymic Stromal Lymphopoietin in Nasal Polyp Gland Duct Epithelium in Patients With Chronic Rhinosinusitis. Am J Rhinol Allergy. 33, 378-387 (2019). 30873846

• Description:
  View product citations for IL25 Antibody - middle region (OAAB10988) on CiteAb

Target Info

• Gene Symbol: IL25
• Alias Symbols: IL17E
• NCBI Gene Id: 64806
• Protein Name: Interleukin-25
• Description of Target: The protein encoded by this gene is a cytokine that shares sequence similarity with interleukin 17. This cytokine can induce NF-kappaB activation, and stimulate the production of interleukin 8. Both this cytokine and interleukin 17B are ligands for the cytokine receptor IL17BR. Studies of a similar gene in mice suggest that this cytokine may be a pro-inflammatory cytokine favoring the Th2-type immune response. Alternative splicing results in multiple transcript variants.
• Uniprot ID: Q9H293
• Protein Accession #: NP_073626.1, NP_758525.1
• Molecular Weight: 20 kDa