Catalog No: OKCD07968
Size:96WELLS
Price: $800.00
SKU
OKCD07968
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Datasheets/ManualsPrintable datasheet for IL24 ELISA Kit (Human) (OKCD07968)
Product Info
Predicted Species ReactivityHomo sapiens|Human
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric
ELISA Kit Duration3h
ELISA Kit PrincipleThe test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Interleukin 24 (IL24). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Interleukin 24 (IL24). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Interleukin 24 (IL24), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Interleukin 24 (IL24) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
ELISA Kit Range15.6-1,000pg/mL
ELISA Kit ReproducibilityIntra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Interleukin 24 (IL24) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Interleukin 24 (IL24) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
ELISA Kit Component
ComponentAmount
IL24 Microplate96 Wells (12 x 8 Well strips)
IL24 Lyophilized Standard2
100X IL24 HRP-Detector Anitbody1 x 120 uL
100X Avidin-HRP Conjugate1 x 120 uL
Standard Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
30X Wash Buffer1 x 20 mL
TMB Substrate1 x 2 mL
Stop Solution1 x 20 mL
Reconstitution and Storage2°C to 8°C|-20°C
Sample TypeSerum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Sensitivity< 3.2pg/mL
Gene SymbolIL24
Gene Full Nameinterleukin 24
Alias SymbolsC49A;FISP;IL10B;IL-4-induced secreted protein;interleukin-24;MDA7;melanocyte-associated Mda-7;melanoma differentiation-associated gene 7 protein;MOB5;ST16;suppression of tumorigenicity 16 (melanoma differentiation);Suppression of tumorigenicity 16 protein.
NCBI Gene Id11009
Protein NameInterleukin-24
Description of TargetHas antiproliferative properties on melanoma cells and may contribute to terminal cell differentiation.
Uniprot IDQ13007
Protein Accession #NP_001172085.1
Nucleotide Accession #NM_001185156.1
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