IL24 Chemi-Luminescent ELISA Kit (Rat) (OKCD03656)

Catalog No: OKCD03656

Size:96WELLS

Rat IL24 ELISA Kit Standard Curve

Product Info

• Predicted Species Reactivity: Rat|Rattus norvegicus
• Application: Chemiluminescent immunoassay
• ELISA Kit Detection Method: Chemiluminescent
• ELISA Kit Duration: 2h, 40min
• ELISA Kit Linearity:

  Sample	1:2	1:4	1:8	1:16
  Serum (n=5)	94-101%	81-101%	90-97%	84-91%
  EDTA Plasma (n=5)	95-102%	84-91%	82-91%	83-93%
  Heparin Plasma (n=5)	89-96%	98-105%	99-105%	92-101%

• ELISA Kit Principle: The microplate provided in this kit has been pre-coated with an antibody specific to Interleukin 24 (IL24). Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to Interleukin 24 (IL24). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is proportional to the Interleukin 24 (IL24) level in the sample or standard.
• ELISA Kit Range: 1.37-1000pg/mL
• ELISA Kit Recovery:

  Matrix	Recovery range (%)	Average(%)
  Serum (n=5)	83-91	87
  EDTA Plasma (n=5)	81-97	91
  Heparin Plasma (n=5)	87-101	92

• ELISA Kit Reproducibility: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Interleukin 24 (IL24) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Interleukin 24 (IL24) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
• ELISA Kit Component:
  • Il24 Microplate: 96 Wells (12 x 8 Well strips)
  • Il24 Lyophilized Standard: 2
  • 100X Il24 Biotinylated Detector Anitbody: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Standard Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 30X Wash Buffer: 1 x 20 mL
  • 100X Luminol Substrate: 1 x 2 mL
  • Substrate Diluent: 1 x 20 mL
• Reconstitution and Storage: 2°C to 8°C|-20°C
• Sample Type: Serum, plasma and other biological fluids
• Sensitivity: 15.6 pg/mL
• Assay Info: Assay Methodology: Quantitative Sandwich ELISA

Target Info

• Gene Symbol: Il24
• Gene Full Name: interleukin 24
• Alias Symbols: cytokine-like protein Mob-5;If2e;IL-24;interferon 2e;interleukin-24;Mda7;Mda-7.
• NCBI Gene Id: 170819
• Protein Name: Interleukin-24
• Description of Target: Has antiproliferative properties on melanoma cells and may contribute to terminal cell differentiation.
• Uniprot ID: Q9JI24
• Protein Accession #: NP_579845
• Nucleotide Accession #: NM_133311