IL24 Chemi-Luminescent ELISA Kit (Human) (OKCD03657)

Catalog No: OKCD03657

Size:96WELLS

Human IL24 ELISA Kit Standard Curve

Product Info

• Predicted Species Reactivity: Homo sapiens|Human
• Application: Chemiluminescent immunoassay
• ELISA Kit Detection Method: Chemiluminescent
• ELISA Kit Duration: 2h, 40min
• ELISA Kit Linearity:

  Sample	1:2	1:4	1:8	1:16
  Serum (n=5)	89-96%	85-102%	81-101%	79-99%
  EDTA Plasma (n=5)	89-98%	90-98%	95-102%	84-96%
  Heparin Plasma (n=5)	79-94%	91-105%	87-95%	79-96%

• ELISA Kit Principle: The microplate provided in this kit has been pre-coated with an antibody specific to Interleukin 24 (IL24). Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to Interleukin 24 (IL24). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is proportional to the Interleukin 24 (IL24) level in the sample or standard.
• ELISA Kit Range: 1.37-1,000pg/mL
• ELISA Kit Recovery:

  Matrix	Recovery range (%)	Average(%)
  Serum (n=5)	97-105	101
  EDTA Plasma (n=5)	98-105	102
  Heparin Plasma (n=5)	85-97	94

• ELISA Kit Reproducibility: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Interleukin 24 (IL24) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Interleukin 24 (IL24) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
• ELISA Kit Component:
  • IL24 Microplate: 96 Wells (12 x 8 Well strips)
  • IL24 Lyophilized Standard: 2
  • 100X IL24 Biotinylated Detector Anitbody: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Standard Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 30X Wash Buffer: 1 x 20 mL
  • 100X Luminol Substrate: 1 x 2 mL
  • Substrate Diluent: 1 x 20 mL
• Reconstitution and Storage: 2°C to 8°C|-20°C
• Sample Type: Serum, plasma and other biological fluids
• Sensitivity: 15.6 pg/mL
• Assay Info: Assay Methodology: Quantitative Sandwich ELISA

Target Info

• Gene Symbol: IL24
• Gene Full Name: interleukin 24
• Alias Symbols: C49A;FISP;IL10B;IL-4-induced secreted protein;interleukin-24;MDA7;melanocyte-associated Mda-7;melanoma differentiation-associated gene 7 protein;MOB5;ST16;suppression of tumorigenicity 16 (melanoma differentiation);Suppression of tumorigenicity 16 protein.
• NCBI Gene Id: 11009
• Protein Name: Interleukin-24
• Description of Target: Has antiproliferative properties on melanoma cells and may contribute to terminal cell differentiation.
• Uniprot ID: Q13007
• Protein Accession #: NP_001172085
• Nucleotide Accession #: NM_001185156