IL23A ELISA Kit (Human) (OKCD06279)

Catalog No: OKCD06279

Size:96WELLS

IL23A ELISA Kit (Human) (OKCD06279) Standard Curve

Product Info

• Predicted Species Reactivity: Homo sapiens|Human
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric
• ELISA Kit Duration: 3h
• ELISA Kit Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Interleukin 23 Subunit Alpha (IL23a). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Interleukin 23 Subunit Alpha (IL23a). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Interleukin 23 Subunit Alpha (IL23a), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Interleukin 23 Subunit Alpha (IL23a) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
• ELISA Kit Range: 7.8-500pg/mL
• ELISA Kit Reproducibility: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Interleukin 23 Subunit Alpha (IL23a) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Interleukin 23 Subunit Alpha (IL23a) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
• ELISA Kit Component:
  • IL23A Microplate: 96 Wells (12 x 8 Well strips)
  • IL23A Lyophilized Standard: 2
  • 100X IL23A HRP-Detector Anitbody: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Standard Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 30X Wash Buffer: 1 x 20 mL
  • TMB Substrate: 1 x 9 mL
  • Stop Solution: 1 x 6 mL
• Reconstitution and Storage: 2°C to 8°C|-20°C
• Sample Type: Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
• Sensitivity: < 2.8pg/mL

Target Info

• Gene Symbol: IL23A
• Gene Full Name: interleukin 23 subunit alpha
• Alias Symbols: IL-23;IL-23 subunit alpha;IL-23A;IL-23-A;IL-23p19;IL23P19;interleukin 23 p19 subunit;interleukin 23, alpha subunit p19;interleukin-23 subunit alpha;interleukin-23 subunit p19;interleukin-six, G-CSF related factor;JKA3 induced upon T-cell activation;P19;SGRF.
• NCBI Gene Id: 51561
• Protein Name: Interleukin-23 subunit alpha
• Description of Target: Associates with IL12B to form the IL-23 interleukin, a heterodimeric cytokine which functions in innate and adaptive immunity. IL-23 may constitute with IL-17 an acute response to infection in peripheral tissues. IL-23 binds to a heterodimeric receptor complex composed of IL12RB1 and IL23R, activates the Jak-Stat signaling cascade, stimulates memory rather than naive T-cells and promotes production of proinflammatory cytokines. IL-23 induces autoimmune inflammation and thus may be responsible for autoimmune inflammatory diseases and may be important for tumorigenesis.
• Uniprot ID: Q9NPF7
• Protein Accession #: NP_057668.1
• Nucleotide Accession #: NM_016584.2