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Catalog No: OKEH00408
Size:96T
Price: $500.00
SKU
OKEH00408
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IL23A ELISA Kit (Human) : 96 Wells (OKEH00408)

Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Please click here to view the MSDS.
Product Info
Predicted Species ReactivityHomo sapiens, Human
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric, OD 450 nm
ELISA Kit Duration~ 3 Hours
ELISA Kit PrincipleAviva Systems Biology IL23A ELISA Kit (Human) (OKEH00408) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Human IL23A has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. After washing,a biotinylated detector antibody specific for Human IL23A is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away.An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Human IL23A captured in well.
ELISA Kit Range31.2-2000pg/mL
ELISA Kit RecoveryMean recovery when spiking into sample matrices at concentrations within the dynamic range: 101%
ELISA Kit ReproducibilityMean Intra-assay CV%: < 6.1% (n = 20)
Mean Inter-assay CV%: < 8.2% (n = 20)
ELISA Kit Component
ComponentAmount
Anti-IL23A Microplate96 Wells (12 x 8 Well strips)
IL23A Lyophilized Standard2
Sample Diluent1 x 20 mL
100X IL23A-Biotin Complex1 x 60 uL
100X Avidin-HRP Conjugate1 x 120 uL
Biotin Complex Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
25X Wash Buffer1 x 30 mL
TMB Substrate1 x 10 mL
Stop Solution1 x 10 mL
Reconstitution and StorageStore as indicated in product manual.
Sample TypeSerum, Plasma, Tissue Homogenates, Cell Culture Supernates, Other Biological Fluids
Sensitivity7.8 pg/mL
SpecificityNatural and recombinant Human Interleukin-23 subunit alpha
Assay InfoAssay Methodology: Quantitative Sandwich ELISA
Gene SymbolIL23A
Gene Full Nameinterleukin 23 subunit alpha
Alias SymbolsIL-23;IL-23 subunit alpha;IL-23A;IL-23-A;IL-23p19;IL23P19;interleukin 23 p19 subunit;interleukin 23, alpha subunit p19;interleukin-23 subunit alpha;interleukin-23 subunit p19;interleukin-six, G-CSF related factor;JKA3 induced upon T-cell activation;P19;SGRF.
NCBI Gene Id51561
Protein NameQ9NPF7
Description of TargetInterleukin-23 subunit alpha
Uniprot IDhttps://www.uniprot.org/uniprot/Q9NPF7
Protein Accession #NP_057668.1
Nucleotide Accession #NM_016584.2
Protein Size (# AA)189
Molecular Weight20 kDa
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