IL22RA2 ELISA Kit (Rat) (OKCD00947)

Catalog No: OKCD00947 (Formerly GWB-KBBUQ7)

Size:96WELLS

Rat Il22ra2 ELISA Kit standard curve

Product Info

• Predicted Species Reactivity: Rat|Rattus norvegicus
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric
• ELISA Kit Duration: 3h
• ELISA Kit Linearity:

  Sample	1:2 Dilution	1:4 Dilution	1:8 Dilution	1:16 Dilution
  serum(n=5)	78-104%	86-101%	90-98%	82-91%
  EDTA plasma(n=5)	83-96%	92-101%	95-104%	95-105%
  heparin plasma(n=5)	87-102%	80-97%	99-105%	87-101%

• ELISA Kit Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Interleukin 22 Receptor Alpha 2 (IL22Ra2). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Interleukin 22 Receptor Alpha 2 (IL22Ra2). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Interleukin 22 Receptor Alpha 2 (IL22Ra2), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Interleukin 22 Receptor Alpha 2 (IL22Ra2) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
• ELISA Kit Range: 0.312-20ng/mL
• ELISA Kit Recovery:

  Matrix	Recovery Range (%)	Average (%)
  serum(n=5)	84-96	87
  EDTA plasma(n=5)	85-94	91
  heparin plasma(n=5)	86-101	97

• ELISA Kit Reproducibility: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Interleukin 22 Receptor Alpha 2 (IL22Ra2) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Interleukin 22 Receptor Alpha 2 (IL22Ra2) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
• ELISA Kit Component:
  • Anti-Il22ra2 Microplate: 96 Wells (12 x 8 Well Strips)
  • Il22ra2 Lyophilized Standard: 2 x 20 ng
  • 100X Biotinylated Il22ra2 Detector Antibody: 120 uL
  • Avidin/HRP Conjugate: 120 uL
  • Standard Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 30X Wash Buffer: 1 x 20 mL
  • TMB Substrate: 1 x 9 mL
  • Stop Solution: 1 x 6 mL
• Reconstitution and Storage: 2°C to 8°C|-20°C
• Sample Type: Serum, plasma, tissue homogenates and other biological fluids.
• Sensitivity: < 0.105 ng/mL
• Specificity: This assay has high sensitivity and excellent specificity for detection of Interleukin 22 Receptor Alpha 2 (IL22Ra2). No significant cross-reactivity or interference between Interleukin 22 Receptor Alpha 2 (IL22Ra2) and analogues was observed.
• Assay Info: Assay Methodology: Quantitative Sandwich Immunoassay

Target Info

• Gene Symbol: Il22ra2
• Gene Full Name: interleukin 22 receptor subunit alpha 2
• Alias Symbols: Crf2-s1;cytokine receptor family II soluble 1;cytokine receptor family type 2, soluble 1;IL-22 receptor subunit alpha-2;IL22BP;IL-22BP;IL-22RA2;IL-22R-alpha-2;interleukin 22 receptor, alpha 2;interleukin-22 receptor subunit alpha-2;interleukin-22-binding protein.
• NCBI Gene Id: 444986
• Protein Name: Interleukin-22 receptor subunit alpha-2
• Description of Target: Receptor for IL22. Binds to IL22, prevents interaction with the functional IL-22R complex and blocks the activity of IL22 (in vitro). May play an important role as an IL22 antagonist in the regulation of inflammatory responses.
• Uniprot ID: Q7TNI4
• Protein Accession #: NP_001003404
• Nucleotide Accession #: NM_001003404.1
• Protein Size (# AA): 229