IL22 ELISA Kit (Human) : 96 Wells (OKAA00020-96W)

Catalog No: OKAA00020-96W

Size:96 Wells

Standard curve of IL-22 protein using OKAA00020_96W

Product Info

• Predicted Species Reactivity: Human
• Application: ELISA
• ELISA Kit Detection Method: Colorimetric, OD450 nm
• ELISA Kit Reproducibility: The coefficient of variation of both intra-assay and inter-assay were less than 10%.
• ELISA Kit Component:

  Components
  2 vials human IL-22 Standard lyophilized, 2000 pg/ml upon reconstitution
  2 vials concentrated Biotin-Conjugate anti-human IL-22 monoclonal antibody
  2 vials Streptavidin-HRP solution,
  1 bottle Standard /sample Diluent
  1 bottle Biotin-Conjugate antibody Diluent
  1 bottle Streptavidin-HRP Diluent
  1 bottle Wash Buffer Concentrate 20x (PBS with 1% Tween-20)
  1 vial Substrate Solution
  1 vial Stop Solution
  2 vials human IL-32alpha Standard lyophilized, 500 pg/ml upon reconstitution
  2 vials concentrated Biotin-Conjugate anti-human IL-32alpha monoclonal antibody

• Additional Information: Sample Type: cell culture supernates, serum, and plasma
  Reagents: REAGENTS
  1. Aluminum pouches with a Microwell Plate coated with monoclonal antibody to human IL-22 (8x12)
  2. 2 vials human IL-22 Standard lyophilized, 2000 pg/ml upon reconstitution
  3. 2 vials concentrated Biotin-Conjugate anti-human IL-22 monoclonal antibody
  4. 2 vials Streptavidin-HRP solution,
  5. 1 bottle Standard /sample Diluent
  6. 1 bottle Biotin-Conjugate antibody Diluent
  7. 1 bottle Streptavidin-HRP Diluent
  8. 1 bottle Wash Buffer Concentrate 20x (PBS with 1% Tween-20)
  9. 1 vial Substrate Solution
  10. 1 vial Stop Solution
  11. 3 pieces Adhesive Films
  12. package insert
• Sample Type: cell culture, supernates, serum, and plasma
• Sensitivity: 15 pg/ml
• Predicted Homology Based on Immunogen Sequence: Human
• Specificity: This assay recognizes both natural and recombinant human IL-21. The factors listed below were prepared at 50ng/ml in Standard /sample Diluent and assayed for cross-reactivity and no significant cross-reactivity or interference was observed.
  Recombinant human: IL-1a, IL-1ra, IL-1 sRI, IL-1 sRII
  Recombinant mouse: IL-1a
  Recombinant porcine: IL-1b
• Assay Info: Assay Method: Quantitative Sandwich ELISA

Target Info

• Gene Symbol: IL22
• Gene Full Name: interleukin 22
• Alias Symbols: TIFa, IL-21, IL-22, ILTIF, IL-TIF, IL-D110, zcyto18, TIFIL-23
• NCBI Gene Id: 50616
• Protein Name: Interleukin-22
• Uniprot ID: Q9GZX6
• Protein Accession #: NP_065386.1
• Nucleotide Accession #: NM_020525.4