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Catalog No: OKEH04403
Size:96T
Price: $525.00
SKU
OKEH04403
Availability: Domestic: within 1-2 weeks delivery | International: 1-2 weeks
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  • $55: Antibody & Protein in US
  • $55 + $25/Kit in US
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Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Product Info
Predicted Species ReactivityHomo sapiens, Human
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric, OD450 nm
ELISA Kit Duration~ 3 Hours
ELISA Kit PrincipleAviva Systems Biology IL20RA ELISA Kit (Human) (OKEH04403) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for IL20RA has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. After washing, a biotinylated detector antibody specific for IL20RA is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away.An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP to generating a blue color product that changes to yellow after adding acidicstop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample IL20RA captured in the well.
ELISA Kit Range0.312-20ng/mL
ELISA Kit RecoveryMean recovery when spiking into sample matrices at concentrations within the dynamic range: 97%
ELISA Kit ReproducibilityMean Intra-assay CV%: <= 3.9% (n = 20)
Mean Inter-assay CV%: <= 7.6% (n = 20)
ELISA Kit Component
ComponentAmount
Anti-IL20RA Microplate96 Wells (12 x 8 Well Strips)
IL20RA Lyophilized Standard2 x 2 ng
Sample Diluent1 x 20 mL
IL20RA Biotinylated Detector Antibody1 x 120 uL
Detector Antibody Diluent1 x 12 mL
HRP Avidin Conjugate1 x 120 uL
Conjugate Diluent1 x 12 mL
25X Wash Buffer1 x 30 mL
TMB Substrate1 x 10 mL
Stop Solution1 x 10 mL
Reconstitution and StorageStore as indicated in product manual.
Sample TypeSerum, Plasma, Tissue Homogenates, Cell Culture Supernates, Other Biological Fluids
Sensitivity0.1 ng/mL
SpecificityNatural and recombinant Human Interleukin-20 receptor subunit alpha
Assay InfoAssay Methodology: Quantitative Sandwich ELISA
Gene SymbolIL20RA
Gene Full Nameinterleukin 20 receptor subunit alpha
Alias Symbolsclass II cytokine receptor ZCYTOR7;CRF2-8;Cytokine receptor class-II member 8;cytokine receptor family 2 member 8;IL-20R1;IL-20RA;IL-20R-alpha;interleukin 20 receptor alpha subunit;interleukin 20 receptor, alpha;interleukin-20 receptor I;interleukin-20 receptor subunit alpha;ZcytoR7.
NCBI Gene Id53832
Protein NameQ9UHF4
Description of TargetInterleukin-20 receptor subunit alpha
Uniprot IDhttps://www.uniprot.org/uniprot/Q9UHF4
Protein Accession #NP_001265651.1
Nucleotide Accession #NM_001278722.1
Protein Size (# AA)553
Molecular Weight62 KDa
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