Catalog No: OKCA02236
Size:96 Wells
Price: $450.00
SKU
OKCA02236
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Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Product Info
Predicted Species ReactivityRat
ELISA Kit Detection MethodColorimetric, OD450 nm
ELISA Kit Duration~ 3.5 Hours
ELISA Kit Linearity
Sample TypeDilutionMean (%)Range (%)
Serum (n=4)Neat9287-98
Serum (n=4)1:29891-104
Serum (n=4)1:48984-99
Serum (n=4)1:89692-100
ELISA Kit PrincipleAviva Systems Biology IL2 ELISA Kit (Rat) (OKCA02236) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for IL2 has been pre-coated onto a 96-wellplate (12 x 8 Well Strips). Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for IL2 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration is read by absorbance at 450 nm and is quantitatively proportional to the amount of sample IL2 captured in well.
ELISA Kit Range6.25 pg/mL-400 pg/mL
ELISA Kit Recovery
Sample TypeMean (%)Range (%)
Serum (n=5)9085-96
ELISA Kit ReproducibilityMean Intra-Assay Variation <10% CV (n=20)
Mean Inter-Assay Variation <12% CV (n=8)
ELISA Kit Component
ComponentAmount
Anti-IL2 Microplate96 Wells (12 x 8 Well strips)
IL2 Lyophilized Standard 2 x 400 pg
100X Biotinylated IL2 Detector Antibody1 x 120 uL
100X Avidin-HRP Conjugate1 x 120 uL
Sample Diluent1 x 50 mL
Detector Antibody Diluent1 x 15 mL
Conjugate Diluent1 x 15 mL
25X Wash Buffer1 x 20 mL
Stop Solution1 x 10 mL
TMB Substrate1 x 10 mL
Reconstitution and StorageStore as indicated in product manual.
Sample TypeSerum, Urine And Tissue Homogenates
Sensitivity1.56 pg/mL
Assay InfoAssay Methodology: Quantitative Sandwich ELISA
Gene SymbolIL2
Alias SymbolsTCGF
NCBI Gene Id100302458
Protein NameInterleukin-2
Description of TargetProduced by T-cells in response to antigenic or mitogenic stimulation, this protein is required for T-cell proliferation and other activities crucial to regulation of the immune response. Can stimulate B-cells, monocytes, lymphokine-activated killer cells, natural killer cells, and glioma cells.
Uniprot IDO77620
Protein Accession #NP_001156652
Nucleotide Accession #NM_001163180
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