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Catalog No: OKWB00303
Size:96T
Price: $450.00
SKU
OKWB00303
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Datasheets/ManualsPrintable datasheet for IL2 ELISA Kit (Monkey) (OKWB00303)
Product Info
Predicted Species ReactivityAotus nancymaae|Monkey
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric, OD 450 nm
ELISA Kit Duration~ 3 Hours
ELISA Kit Linearity
Sample Type1:2 Dilution1:4 Dilution1:8 Dilution1:16 Dilution
Serum (n=5)91-105%85-104%87-103%85-97%
EDTA Plasma (n=5)89-99%84-101%83-100%93-101%
Heparin Plasma (n=5)80-98%81-86%81-96%80-99%
ELISA Kit PrincipleAviva Systems Biology IL2 ELISA Kit (Monkey) (OKWB00303) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for IL2 has been pre-coated onto a 96-well plate (12 x 8 Well Strips). Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for IL2 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample IL2 captured in the well.
ELISA Kit Range15.625-1000pg/ml
ELISA Kit Recovery
Sample TypeRangeMean
Serum (n=5)86-10094
EDTA Plasma (n=5)88-10395
Heparin Plasma (n=5)94-103100
ELISA Kit ReproducibilityMean Intra-Assay Precision <10%
Mean Inter-Assay Precision <12%
ELISA Kit Component
ComponentAmount
IL2 Microplate96 Wells (12 x 8 Well strips)
IL2 Lyophilized Standard2 x 1 ng
100X IL2 Biotinylated Detector Anitbody1 x 120 uL
100X Avidin-HRP Conjugate1 x 120 uL
Standard Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
25X Wash Buffer1 x 20 mL
TMB Substrate1 x 10 mL
Stop Solution1 x 10 mL
Reconstitution and StorageStore as indicated in product manual.
Sample TypeSerum, Plasma, Cell Cultures, Tissue Homogenates, Other Biological Fluids
Sensitivity9.4 pg/mL
Assay InfoAssay Type: Quantitative Sandwich ELISA
Gene SymbolIL2
Gene Full Nameinterleukin 2
Alias SymbolsIL-2;interleukin-2;T-cell growth factor;TCGF.
NCBI Gene Id105731298
Protein NameInterleukin-2
Description of TargetCytokine produced by activated CD4-positive helper T-cells and to a lesser extend activated CD8-positive T-cells and natural killer (NK) cells that plays pivotal roles in the immune response and tolerance. Binds to a receptor complex composed of either the high-affinity trimeric IL-2R (IL2RA/CD25, IL2RB/CD122 and IL2RG/CD132) or the low-affinity dimeric IL-2R (IL2RB and IL2RG). Interaction with the receptor leads to oligomerization and conformation changes in the IL-2R subunits resulting in downstream signaling starting with phosphorylation of JAK1 and JAK3. In turn, JAK1 and JAK3 phosphorylate the receptor to form a docking site leading to the phosphorylation of several substrates including STAT5. This process leads to activation of several pathways including STAT, phosphoinositide-3-kinase/PI3K and mitogen-activated protein kinase/MAPK pathways. Functions as a T-cell growth factor and can increase NK-cell cytolytic activity as well. Promotes strong proliferation of activated B-cells and subsequently immunoglobulin production. Plays a pivotal role in regulating the adaptive immune system by controlling the survival and proliferation of regulatory T-cells, which are required for the maintenance of immune tolerance. Moreover, participates in the differentiation and homeostasis of effector T-cell subsets, including Th1, Th2, Th17 as well as memory CD8-positive T-cells.
Uniprot IDQ7JFM5
Protein Accession #XP_007997918
Nucleotide Accession #XM_007999727
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