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Catalog No: OKCD04415
Size:96WELLS
Price: $700.00
SKU
OKCD04415
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Datasheets/ManualsPrintable datasheet for IL2 ELISA Kit (Guinea pig) (OKCD04415)
Product Info
Predicted Species ReactivityCavia porcellus|Guinea Pig
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric
ELISA Kit Duration3h
ELISA Kit Linearity
Sample1:21:41:81:16
Serum (n=5)96-105%86-96%78-97%78-97%
EDTA Plasma (n=5)88-95%79-101%98-105%97-105%
Heparin Plasma (n=5)86-101%80-101%84-93%80-96%
ELISA Kit PrincipleThe test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Interleukin 2 (IL2). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Interleukin 2 (IL2). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Interleukin 2 (IL2), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Interleukin 2 (IL2) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
ELISA Kit Range15.6-1,000pg/mL
ELISA Kit Recovery
MatrixRecovery range (%)Average (%)
Serum (n=5)81-10594
EDTA Plasma (n=5)83-10390
Heparin Plasma (n=5)90-9995
ELISA Kit ReproducibilityIntra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Interleukin 2 (IL2) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Interleukin 2 (IL2) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
ELISA Kit Component
ComponentAmount
IL2 Microplate96 Wells (12 x 8 Well strips)
IL2 Lyophilized Standard2
100X Biotynilated IL2 Detector Anitbody1 x 120 uL
100X Avidin-HRP Conjugate1 x 120 uL
Sample Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
30X Wash Buffer1 x 20 mL
Stop Solution1 x 6 mL
TMB Substrate 1 x 9 mL
Reconstitution and Storage2°C to 8°C|-20°C
Sample TypeSerum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.
Sensitivity5.5 pg/mL
Assay InfoAssay Methodology: Quantitative Sandwich ELISA
Gene SymbolIl2
Gene Full Nameinterleukin 2
Alias Symbolsinterleukin-2.
NCBI Gene Id100379540
Protein NameInterleukin-2
Description of TargetProduced by T-cells in response to antigenic or mitogenic stimulation, this protein is required for T-cell proliferation and other activities crucial to regulation of the immune response.
Uniprot IDO88210
Protein Accession #NP_001166308.1
Nucleotide Accession #NM_001172837.1
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