IL2 ELISA Kit (Goat) (OKCA00589)

Catalog No: OKCA00589

Size:96 Wells

Goat IL2 ELISA Kit standard curve

Product Info

• Predicted Species Reactivity: Goat
• ELISA Kit Detection Method: Colorimetric, OD450 nm
• ELISA Kit Duration: ~ 3.5 Hours
• ELISA Kit Linearity:

  Dilution	Mean n=4 (%)	Range (%)
  Neat	90	85-95
  1:2	98	94-102
  1:4	102	94-107
  1:8	94	87-98

• ELISA Kit Principle: Aviva Systems Biology IL2 ELISA Kit (Goat) (OKCA00589) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for IL2 has been pre-coated onto a 96-well plate (12 x 8 Well Strips). Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for IL2 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm and is quantitatively proportional to the amount of sample IL2 captured in the well.
• ELISA Kit Range: 15.6 pg/mL-1,000 pg/mL
• ELISA Kit Recovery:

  Matrix	Recovery range (%)	Average(%)
  Serum (n=5)	97	95-102
  EDTA Plasma (n=4)	93	88-97

• ELISA Kit Reproducibility: Intra-assay Precision: 3 samples with known low, middle and high level IL2 were tested with 20 replicates across one plate.
  Inter-assay Precision: 3 samples with known low, middle and high level IL2 were tested on three different plates with 8 replicates, respectively.
  Intra-Assay CV: <15%
  Inter-Assay CV: <15%
• ELISA Kit Component:
  • IL2 Microplate: 96 Wells (12 x 8 Well strips)
  • IL2 Lyophilized Standard: 2
  • 100X Biotinylated IL2 Detector Anitbody: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Sample Diluent: 1 x 50 mL
  • Detector Antibody Diluent: 1 x 15 mL
  • Conjugate Diluent: 1 x 15 mL
  • 25X Wash Buffer: 1 x 20 mL
  • Stop Solution: 1 x 10 mL
  • TMB Substrate: 1 x 10 mL
• Reconstitution and Storage: Store as indicated in product manual.
• Sample Type: Serum, Plasma, Tissue Homogenates
• Sensitivity: 3.9 pg/mL
• Assay Info: Assay Methodology: Quantitative Sandwich ELISA

Target Info

• Gene Symbol: IL2
• Gene Full Name: Interleukin-2
• Protein Name: Interleukin-2
• Description of Target: Produced by T-cells in response to antigenic or mitogenic stimulation, this protein is required for T-cell proliferation and other activities crucial to regulation of the immune response. Can stimulate B-cells, monocytes, lymphokine-activated killer cells, natural killer cells, and glioma cells.
• Uniprot ID: P36835
• Protein Accession #: NP_001274496.1
• Nucleotide Accession #: NM_001287567.1