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IL2 Antibody (OASA07877)

0.1 mg
$609.00
In Stock
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Gene Symbol:
IL2
NCBI Gene Id:
3558
Protein Name:
Interleukin-2
Swissprot Id:
P60568
Protein Accession #:
NP_000577.2
Alias Symbols:
IL-2, TCGF, lymphokine, IL2
Replacement Item:
This antibody may replace item sc-133118 from Santa Cruz Biotechnology.
Description of Target:
RABBIT ANTI HUMAN INTERLEUKIN-2
Protein Size (# AA):
153
Host:
Rabbit
Clonality:
Polyclonal
Application:
ELISA, WB
Tissue Tool:
Find tissues and cell lines supported by DNA array analysis to express IL2.
RNA Seq:
Find tissues and cell lines supported by RNA-seq analysis to express IL2.
Immunogen:
The immunogen for anti-IL2 antibody: recombinant human IL-2 (PHP042A)
Predicted Species Reactivity:
Human
Predicted Homology Based on Immunogen Sequence:
Human
Product Format:
Purified IgG - lyophilised
Reconstitution and Storage:
Reconstitution: Reconstitute with 0.1ml distilled water
Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Aviva Systems Biology recommend that the vial is gently mixed after reconstitution. For l
Datasheets/Manuals:
Printable datasheet for anti-IL2 antibody - OASA07877
Specificity:
IL-2
Isotype:
Polyclonal IgG
Application Info:
This product requires antigen retrieval using heat treatment prior to staining of paraffin sections. Sodium citrate buffer pH 6.0 is recommended for this purpose.
:::
Preservative Stabilisers: None present
Antiserum Preparation: Antisera to human IL-2 were raised by repeated immunisations of rabbits with highly purified antigen. Purified IgG prepared by affinity chromatography.
:::
Approx Protein Conc: IgG concentration 1.0mg/ml after reconstitution.
Buffer Solutions: Phosphate buffered saline pH7.4
Protocol Information:
Citation: 1: Morikawa K, Oseko F, Morikawa S, Iwamoto K. Immunomodulatory effects of three macrolides, midecamycin acetate, josamycin, and clarithromycin, on humanT-lymphocyte function in vitro. Antimicrob Agents Chemother. 1994 Nov;38(11):2643-7. PubMed PMID: 7532933; PubMed Central PMCID: PMC188255.
Species: Human
Experiment Name: Assessment of IL-2 activity in culture supernatants.
Experiment Background: Effect of macrolides on IL-2 production.
Experimental Steps: 1. IL-2 concentration in culture supernatant was determined with commercial enzyme-linked immunosorbent assay (ELISA) kits (Oxford, England). 2. IL-2 content was calculated with a standard curve derived by linear dilution of the cytokine standards supplied with the respective kits. 3. The minimal sensitivity of IL-2 determination in this assay is 67 µg/ml.
Other Reagents Used: 2-aminoethylisothiouronium bromide
Number Of Protocols: 1
Target Reference:
1. Fujita, T. et al. (1983) Structure of the human Interleukin 2 gene. P.N.A.S. 80: 7437-7441.
2. Nelson, B.H. (2004) IL-2, regulatory T cells, and tolerance. J. Immunol. 172: 3983-3988.

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