Catalog No: OKEH03822
Size:96T
Price: $625.00
SKU
OKEH03822
Availability: Domestic: within 1-2 weeks delivery | International: 1-2 weeks
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  • $55: Antibody & Protein in US
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Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Product Info
Predicted Species ReactivityPorcine, Sus scrofa
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric, OD450 nm
ELISA Kit Duration~ 3 Hours
ELISA Kit PrincipleAviva Systems Biology IL1RN ELISA Kit (Pig) (OKEH03822) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for IL1RN has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for IL1RN is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away.An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidicstop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample IL1RN captured in well.
ELISA Kit Range0.156-10ng/mL
ELISA Kit Component
ComponentAmount
Anti-IL1RN Microplate96 Wells (12 x 8 Well strips)
IL1RN Lyophilized Standard2
Sample Diluent1 x 20 mL
100X Biotinylated IL1RN Detector Antibody1 x 120 uL
100X Avidin-HRP Conjugate1 x 120 uL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
25X Wash Buffer1 x 30 mL
TMB Substrate1 x 10 mL
Stop Solution1 x 10 mL
Reconstitution and StorageStore as indicated in product manual.
Sample TypeSerum, Plasma, Tissue Homogenates , Cell Culture Supernates, Other Biological Fluids
Sensitivity0.06 ng/mL
SpecificityNatural and recombinant Pig Interleukin-1 receptor antagonist protein
Assay InfoAssay Methodology: Quantitative Sandwich ELISA
Gene SymbolIL1RN
Gene Full Nameinterleukin 1 receptor antagonist
Alias SymbolsIL1 inhibitor;IL-1ra;IL-1RN;interleukin-1 receptor antagonist protein;IRAP;IRAP1.
NCBI Gene Id397499
Protein NameQ29056
Description of TargetInterleukin-1 receptor antagonist protein
Uniprot IDhttps://www.uniprot.org/uniprot/Q29056
Protein Accession #NP_999427.1
Nucleotide Accession #NM_214262.1
Protein Size (# AA)177
Molecular Weight20 kDa
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