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Catalog No: OKCD00548 (Formerly GWB-KBBUF8)
Size:96WELLS
Price: $675.00
SKU
OKCD00548
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Datasheets/ManualsPrintable datasheet for IL1RAPL2 ELISA Kit (Human) (OKCD00548)
Product Info
Predicted Species ReactivityHomo sapiens|Human
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric
ELISA Kit Duration3h
ELISA Kit PrincipleThe test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Interleukin 1 Receptor Accessory Protein Like Protein 2 (IL1RAPL2). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Interleukin 1 Receptor Accessory Protein Like Protein 2 (IL1RAPL2). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Interleukin 1 Receptor Accessory Protein Like Protein 2 (IL1RAPL2), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Interleukin 1 Receptor Accessory Protein Like Protein 2 (IL1RAPL2) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
ELISA Kit Range0.156-10ng/mL
ELISA Kit ReproducibilityIntra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Interleukin 1 Receptor Accessory Protein Like Protein 2 (IL1RAPL2) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Interleukin 1 Receptor Accessory Protein Like Protein 2 (IL1RAPL2) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
ELISA Kit Component
ComponentAmount
Anti-IL1RAPL2 Microplate96 Wells (12 x 8 Well Strips)
IL1RAPL2 Lyophilized Standard2 x 10 ng
100X Biotinylated IL1RAPL2 Detector Antibody120 uL
Avidin/HRP Conjugate120 uL
Standard Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
30X Wash Buffer1 x 20 mL
TMB Substrate1 x 9 mL
Stop Solution1 x 6 mL
Reconstitution and Storage2°C to 8°C|-20°C
Sample TypeTissue homogenates and other biological fluids.
Sensitivity< 0.054 ng/mL
SpecificityThis assay has high sensitivity and excellent specificity for detection of Interleukin 1 Receptor Accessory Protein Like Protein 2 (IL1RAPL2). No significant cross-reactivity or interference between Interleukin 1 Receptor Accessory Protein Like Protein 2 (IL1RAPL2) and analogues was observed.
Assay InfoAssay Methodology: Quantitative Sandwich Immunoassay
Gene SymbolIL1RAPL2
Gene Full Nameinterleukin 1 receptor accessory protein like 2
Alias SymbolsIL-1 receptor accessory protein-like 2;IL1R9;IL-1R9;IL-1R-9;IL1RAPL-2;IL1RAPL-2-related protein;interleukin 1 receptor 9;Interleukin-1 receptor 9;three immunoglobulin domain-containing IL-1 receptor-related 1;TIGIRR-1;X-linked interleukin-1 receptor accessory protein-like 2.
NCBI Gene Id26280
Protein NameX-linked interleukin-1 receptor accessory protein-like 2
Description of TargetX-linked interleukin-1 receptor accessory protein-like 2
Uniprot IDQ9NP60
Protein Accession #NP_059112
Nucleotide Accession #NM_017416.1
Protein Size (# AA)686
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