Catalog No: OKEH02970
Size:96T
Price: $425.00
SKU
OKEH02970
Availability: Domestic: within 1-2 weeks delivery | International: 1-2 weeks
Please contact customer service when ordering 10+ kits.

Bulk
Order
Aviva's
Satisfaction
Guarantee
Contact Us:
Shipping Info:
  • $55: Antibody & Protein in US
  • $55 + $25/Kit in US
  • Contact us for international orders.
Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Product Info
Predicted Species ReactivityMouse, Mus musculus
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric, OD450 nm
ELISA Kit Duration~ 3 Hours
ELISA Kit PrincipleAviva Systems Biology IL1R1 ELISA Kit (Mouse) (OKEH02970) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Il1r1 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells and removed. A biotinylated detector antibody specific for Il1r1 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Il1r1 captured in well.
ELISA Kit Range78-5000pg/mL
ELISA Kit RecoveryMean recovery when spiking into sample matrices at concentrations within the dynamic range: 90%
ELISA Kit ReproducibilityMean Intra-assay CV%: <= 6.4% (n = 20)
Mean Inter-assay CV%: <= 8.2% (n = 20)
ELISA Kit Component
ComponentAmount
Anti-Il1r1 Microplate96 Wells (12 x 8 Well strips)
Il1r1 Lyophilized Standard2
100X Biotinylated Il1r1 Detector Antibody1 x 120 uL
100X Avidin-HRP Conjugate1 x 120 uL
Sample Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
25X Wash Buffer1 x 30 mL
TMB Substrate1 x 10 mL
Stop Solution1 x 10 mL
Reconstitution and StorageStore as indicated in product manual.
Sample TypeSerum, Plasma, Tissue homogenates, Cell culture supernates, Other biological fluids
Sensitivity40.1 pg/mL
SpecificityNatural and recombinant Mouse Interleukin-1 receptor type 1
Assay InfoAssay Methodology: Quantitative Sandwich ELISA
Gene SymbolIl1r1
Gene Full Nameinterleukin 1 receptor, type I
Alias SymbolsCD121;CD121 antigen-like family member A;CD121a;CD121b;I;IL-;Il1;IL-1 receptor alpha chain;Il1r-1;IL-1R1;IL-1R-1;IL-1R-alpha;IL-1RT1;IL-1RT-1;IL-iR;interleukin-1 receptor alpha;interleukin-1 receptor type 1;Interleukin-1 receptor type I;p80.
NCBI Gene Id16177
Protein NameP13504
Description of TargetInterleukin-1 receptor type 1
Uniprot IDhttps://www.uniprot.org/uniprot/P13504
Protein Accession #NP_032388.1
Nucleotide Accession #NM_008362.2
Protein Size (# AA)576
Molecular Weight67 kDa
Write Your Own Review
You're reviewing:IL1R1 ELISA Kit (Mouse) (OKEH02970)
Your Rating
We found other products you might like!