Catalog No: OOPA00026 (Formerly GWB-BSP048)
Size:20ug
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Datasheets/Manuals | Printable datasheet for OOPA00026 |
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Product Format | Sterile Filtered White lyophilized (freeze-dried) powder. |
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Host | Escherichia Coli. |
Additional Information | Solubility: It is recommended to quick spin followed by reconstitution of IL1F6 in PBS to a concentration no less than 100 ug/ml, which can then be further diluted to other aqueous solutions. |
:: | Product Introduction: Human interleukin family 1, member 6 (hIL-1F6 / FIL1-epsilon) belongs to the interleukin 1 cytokine family. IL1F6 is expressed in the immune system and the fetal brain, but not in other tissues tested or in multiple hematopoietic cell lines. IL1F6 is the only IL-1 family member found to be expressed on T-cells. IL1F6 functions via receptor complex of IL-1Rrp2 and IL-1RAcP. The recombinant IL1F6, along with IL1F8 and IL1F9, acts as an agonist by activating the pathway involving NF?B and MAPK in an IL-1Rrp2 dependent manner. Product Description: IL1F6 Human Recombinant produced in E.Coli is a single, non-glycosylated, Polypeptide chain containing 158 amino acids and having a molecular mass of 17.7kDa. The IL1F6 is purified by proprietary chromatographic techniques. |
Reconstitution and Storage | Lyophilized IL1F6 although stable at room temperature for 3 weeks, should be stored desiccated below -18C. Upon reconstitution IL1F6 should be stored at 4C between 2-7 days and for future use below -18C. Please prevent freeze-thaw cycles. |
Formulation | IL1F6 was lyophilized after extensive dialysis against 20mM Phosphate buffer, pH7.4. |
Purification | Greater than 95.0% as determined by: (a) Analysis by RP-HPLC. (b) Analysis by SDS-PAGE. |
Purity | Greater than 95.0% as determined by: (a) Analysis by RP-HPLC. (b) Analysis by SDS-PAGE. |
Biological Activity | As measured by its binding ability in a functional ELISA, immobilized IL1F6 at 1 ug/ml (100 ul/well) can bind rHuIL-1 Rrp2/Fc Chimera with a linear range of 0.15- 5 ug/ml. |
Peptide Sequence | The sequence of the first five N-terminal amino acids was determined and was found to be Met-Glu-Lys-Ala-Leu. |
Gene Symbol | IL1F6 |
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Alias Symbols | Interleukin-1 family member 6, IL-1F6, FIL1 epsilon, Interleukin-1 epsilon, IL-1 epsilon, IL1F6, FIL1E, IL1E, MGC129552, MGC129553. |
- Protocol:
- Reconstitution & Storage Instructions
- Western Blotting/Immunoblotting (WB/IB) Protocol
- Immunohistochemistry (IHC) Protocol
- Immunocytochemistry (ICC) Protocol
- Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
- Blocking Peptide Competition Protocol (BPCP)
- Immunoprecipitation (IP) Protocol
- Antibody Array (AA) Protocol
- Tips Information:
-
See our General FAQ page.
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