IL1B ELISA Kit (Rat) (OKBB00177)

Catalog No: OKBB00177

Size:96 Tests

Rat IL1B ELISA Kit standard curve

Product Info

• Predicted Species Reactivity: Rat
• Application: ELISA
• ELISA Kit Detection Method: Colorimetric, OD450 nm
• ELISA Kit Duration: ~ 3 Hours
• ELISA Kit Principle: Aviva's rat IL-1 beta ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for IL-1 beta has been precoated onto 96-well plates. Standards (E.coli,V117-S268) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for IL-1 beta is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The absorbance of yellow is proportional to the rat IL-1 beta amount of sample captured in plate.
• ELISA Kit Range: 31.2 pg/ml - 2,000 pg/ml
• ELISA Kit Reproducibility:

  	Intra-Assay Precision			Inter-Assay Precision
  Sample	1	2	3	1	2	3
  Mean (pg/mL)	105	756	1359	112	688	1452
  St. Dev.	3.99	34.78	66.59	5.712	37.84	92.93
  %CV	3.8	4.60	4.89	5.1	5.5	6.40

• ELISA Kit Component:
  • Lyophilized recombinant rat IL-1β standard: 10 ng/tube x 2
  • Anti-rat IL-1β Antibody Well Plate: 96 Wells
  • Sample diluent buffer: 30 mL
  • Biotinylated anti-rat IL-1β antibody: 130 uL, dilution 1:100
  • Antibody diluent buffer: 12 mL
  • Avidin-Biotin-Peroxidase Complex (ABC): 130 uL, dilution 1:100
  • ABC diluent buffer: 12 mL
  • TMB color developing agent: 10 mL
  • TMB stop solution: 10 mL
• Additional Information: Range: 31.2pg/ml-2000pg/ml
  Principle: Aviva’s rat IL-1β ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for IL-1Β has been precoated onto 96-well plates. Standards (E.coli,V117-S268) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for IL-1Β is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the rat IL-1Β amount of sample captured in plate.
  Notes: 1. To inspect the validity of experiment operation and the appropriateness of sample dilution proportion, pilot
  experiment using standards and a small number of samples is recommended.
  2. The TMB Color Developing agent is colorless and transparent before using, contact us freely if it is not the case.
  3. Before using the Kit, spin tubes and bring down all components to the bottom of tubes.
  4. Duplicate well assay is recommended for both standard and sample testing.
  5. Don’t let 96-well plate dry, for dry plate will inactivate active components on plate.
  6. Don’t reuse tips and tubes to avoid cross contamination.
  7. To avoid to use the reagents from different batches together.
  8. In order to avoid marginal effect of plate incubation due to temperature difference ( reaction may be stronger in the
  marginal wells), it is suggested that the diluted ABC and TMB solution will be pre-warmed in 37C for 30 min before
  using.
  Background: The Interleukin 1 family is a group of 11 beta trefoil cytokines.1 Interleukin-1, produced mainly by blood monocytes, mediates the panoply of host reactions collectively known as acute phase response .2 Interleukin-1beta (IL-1beta) is a potent stimulator of bone resorption, and has been implicated in the pathogenesis of high bone turnover and osteoporosis.3 IL-1beta belongs to proinflammatory cytokines, promote cancer cell adhesion and liver metastases by up-regulating the expression of vascular cell adhesion molecule-1 (VCAM-1) on hepatic sinusoidal endothelium (HSE).4
• Reconstitution and Storage: Store at 4C for 6 months, at -20C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
• Sample Type: cell culture supernates, serum and plasma (heparin, EDTA)
• Sensitivity: <1 pg/ml
• Predicted Homology Based on Immunogen Sequence: No detectable crosss-reactivity with any other cytokine.
• Reference: 1. Vincent T. DeVita; Theodore S. Lawrence; Steven A. Rosenberg; Robert A. Weinberg, Ronald A. DePinho (1 April
  2008). DeVita, Hellman, and Rosenberg's cancer: principles & practice of oncology. Lippincott Williams & Wilkins.
  pp. 506–. ISBN 9780781772075. http://books.google.com/books?id=NF90HzUFPjgC&pg=PA506. Retrieved 3
  December 2010.
  2. March, C. J., Mosley, B., Larsen, A., Cerretti, D. P., Braedt, G., Price, V., Gillis, S., Henney, C. S., Kronheim, S. R.,
  Grabstein, K., Conlon, P. J., Hopp, T. P., Cosman, D. Cloning, sequence and expression of two distinct human
  interleukin-1 complementary DNAs. Nature 315: 641-647, 1985.
  3. Langdahl, B. L., Lokke, E., Carstens, M., Stenkjaer, L. L., Eriksen, E. F. Osteoporotic fractures are associated with
  an 86-base pair repeat polymorphism in the interleukin-1-receptor antagonist gene but not with polymorphisms in
  the interleukin-1 beta gene. J. Bone Miner. Res. 15: 402-414, 2000.
  4. Vidal-Vanaclocha, F., Fantuzzi, G., Mendoza, L., Fuentes, A. M., Anasagasti, M. J., Martin, J., Carrascal, T., Walsh,
  P., Reznikov, L. L., Kim, S.-H., Novick, D., Rubinstein, M., Dinarello, C. A. IL-18 regulates IL-1-beta-dependent
  hepatic melanoma metastasis via vascular cell adhesion molecule-1. Proc. Nat. Acad. Sci. 97: 734-739, 2000.
• Specificity: Natural and recombinant rat IL-1 beta
• Immunogen: Expression system for standard: E.coli; Immunogen sequence: V117-S268
• Description: For quantitative detection of rat IL-1 beta in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).

Target Info

• Gene Symbol: IL1B
• Gene Full Name: interleukin 1, beta
• Alias Symbols: Catabolin, Hematopoietin 1, IFN beta inducing factor, IL 1, IL 1 beta, IL 1B, IL-1 beta, IL1, IL1 BETA, IL1B, IL1B_HUMAN, IL1F2, Interleukin 1 beta, Interleukin 1 beta precursor, Interleukin-1 beta, LAF, OAF, Osteoclast activating factor, Preinterleukin beta, Pro interleukin 1 beta
• NCBI Gene Id: 24494
• Protein Name: Interleukin-1 beta
• Description of Target: The Interleukin 1 family is a group of 11 beta trefoil cytokines.1 Interleukin-1, produced mainly by blood monocytes, mediates the panoply of host reactions collectively known as acute phase response .2 Interleukin-1beta (IL-1beta) is a potent stimulator of bone resorption, and has been implicated in the pathogenesis of high bone turnover and osteoporosis.3 IL-1beta belongs to proinflammatory cytokines, promote cancer cell adhesion and liver metastases by up-regulating the expression of vascular cell adhesion molecule-1 (VCAM-1) on hepatic sinusoidal endothelium (HSE).4
• Uniprot ID: Q63264