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Catalog No: OKCD01524 (Formerly GWB-KBBSV8)
Size:96WELLS
Price: $525.00
SKU
OKCD01524
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Datasheets/ManualsPrintable datasheet for IL1B ELISA Kit (Rabbit) (OKCD01524)
Product Info
Predicted Species ReactivityOryctolagus cuniculus|Rabbit
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric
ELISA Kit Duration3h
ELISA Kit Linearity
Sample1:2 Dilution1:4 Dilution1:8 Dilution1:16 Dilution
Serum (n=5)82-101%78-91%81-102%88-96%
EDTA Plasma (n=5)80-91%80-99%80-91%92-99%
Heparin Plasma (n=5)90-98%95-102%80-92%94-102%
ELISA Kit PrincipleThe test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Interleukin 1 Beta (IL1b). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Interleukin 1 Beta (IL1b). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Interleukin 1 Beta (IL1b), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Interleukin 1 Beta (IL1b) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
ELISA Kit Range7.8-500pg/mL
ELISA Kit Recovery
MatrixRecovery Range (%)Average (%)
Serum (n=5)92-10196
EDTA Plasma (n=5)79-9689
Heparin Plasma (n=5)95-10399
ELISA Kit ReproducibilityIntra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Interleukin 1 Beta (IL1b) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Interleukin 1 Beta (IL1b) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
ELISA Kit Component
ComponentAmount
Anti-IL1B Microplate96 Wells (12 x 8 Well Strips)
IL1B Lyophilized Standard2 x 500 pg
100X Biotinylated IL1B Detector Antibody120 uL
Avidin/HRP Conjugate120 uL
Standard Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
30X Wash Buffer1 x 20 mL
TMB Substrate1 x 9 mL
Stop Solution1 x 6 mL
Reconstitution and Storage2°C to 8°C|-20°C
Sample TypeSerum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Sensitivity< 1.15 pg/mL
SpecificityThis assay has high sensitivity and excellent specificity for detection of Interleukin 1 Beta (IL1b). No significant cross-reactivity or interference between Interleukin 1 Beta (IL1b) and analogues was observed.
Assay InfoAssay Methodology: Quantitative Sandwich Immunoassay
Gene SymbolIL1B
Gene Full Nameinterleukin 1 beta
Alias SymbolsIL-1 beta;IL1-BETA;interleukin-1 beta;lymphocyte proliferation-potentiating factor.
NCBI Gene Id100008990
Protein NameInterleukin-1 beta
Description of TargetPotent proinflammatory cytokine. Initially discovered as the major endogenous pyrogen, induces prostaglandin synthesis, neutrophil influx and activation, T-cell activation and cytokine production, B-cell activation and antibody production, and fibroblast proliferation and collagen production. Promotes Th17 differentiation of T-cells. Synergizes with IL12/interleukin-12 to induce IFNG synthesis from T-helper 1 (Th1) cells.
Uniprot IDP14628
Protein Accession #NP_001075670.1
Nucleotide Accession #NM_001082201.1
Protein Size (# AA)268
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