IL1B ELISA Kit (Guinea pig) (OKCD01523)

Catalog No: OKCD01523 (Formerly GWB-KBBKY5)

Size:96WELLS

Guinea Pig IL1B ELISA Kit Standard Curve

Product Info

• Predicted Species Reactivity: Cavia porcellus|Guinea Pig
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric
• ELISA Kit Duration: 3h
• ELISA Kit Linearity:

  Sample	1:2 Dilution	1:4 Dilution	1:8 Dilution	1:16 Dilution
  Serum (n=5)	86-99%	91-99%	91-101%	98-105%
  EDTA Plasma (n=5)	99-105%	98-105%	79-101%	78-101%
  Heparin Plasma (n=5)	79-96%	78-96%	83-93%	93-101%

• ELISA Kit Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Interleukin 1 Beta (IL1b). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Interleukin 1 Beta (IL1b). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Interleukin 1 Beta (IL1b), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Interleukin 1 Beta (IL1b) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
• ELISA Kit Range: 7.8-500pg/mL
• ELISA Kit Recovery:

  Matrix	Recovery Range (%)	Average (%)
  Serum (n=5)	88-96	92
  EDTA Plasma (n=5)	84-91	88
  Heparin Plasma (n=5)	94-103	97

• ELISA Kit Reproducibility: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Interleukin 1 Beta (IL1b) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Interleukin 1 Beta (IL1b) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
• ELISA Kit Component:
  • Anti-IL1B Microplate: 96 Wells (12 x 8 Well Strips)
  • IL1B Lyophilized Standard: 2 x 500 pg
  • 100X Biotinylated IL1B Detector Antibody: 120 uL
  • Avidin/HRP Conjugate: 120 uL
  • Standard Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 30X Wash Buffer: 1 x 20 mL
  • TMB Substrate: 1 x 9 mL
  • Stop Solution: 1 x 6 mL
• Reconstitution and Storage: 2°C to 8°C|-20°C
• Sample Type: Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
• Sensitivity: < 2.8 pg/mL
• Specificity: This assay has high sensitivity and excellent specificity for detection of Interleukin 1 Beta (IL1b). No significant cross-reactivity or interference between Interleukin 1 Beta (IL1b) and analogues was observed.
• Assay Info: Assay Methodology: Quantitative Sandwich Immunoassay

Target Info

• Gene Symbol: Il1b
• Gene Full Name: interleukin 1 beta
• Alias Symbols: IL-1 beta;interleukin-1 beta.
• NCBI Gene Id: 100135556
• Protein Name: Interleukin-1 beta
• Description of Target: Potent proinflammatory cytokine. Initially discovered as the major endogenous pyrogen, induces prostaglandin synthesis, neutrophil influx and activation, T-cell activation and cytokine production, B-cell activation and antibody production, and fibroblast proliferation and collagen production. Promotes Th17 differentiation of T-cells. Synergizes with IL12/interleukin-12 to induce IFNG synthesis from T-helper 1 (Th1) cells.
• Uniprot ID: Q9WVG1
• Protein Accession #: NP_001166439.1
• Nucleotide Accession #: NM_001172968.1
• Protein Size (# AA): 266