IL1B ELISA Kit (Goat) (OKCA00588)

Catalog No: OKCA00588

Size:96 Wells

Goat IL1B ELISA Kit standard curve

Product Info

• Predicted Species Reactivity: Goat
• ELISA Kit Detection Method: Colorimetric, OD450 nm
• ELISA Kit Duration: ~ 3.5 Hours
• ELISA Kit Linearity:

  Dilution	Mean n=4 (%)	Range (%)
  Neat	87	82-94
  1:2	90	82-97
  1:4	92	85-100
  1:8	95	90-103

• ELISA Kit Principle: Aviva Systems Biology IL1B ELISA Kit (Goat) (OKCA00588) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for IL1B has been pre-coated onto a 96-well plate (12 x 8 Well Strips). Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for IL1B is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm and is quantitatively proportional to the amount of sample IL1B captured in the well.
• ELISA Kit Range: 3.12 pg/mL-200 pg/mL
• ELISA Kit Recovery:

  Matrix	Recovery range (%)	Average(%)
  Serum (n=5)	90	85-98
  EDTA Plasma (n=4)	99	93-108

• ELISA Kit Reproducibility: Intra-assay Precision: 3 samples with known low, middle and high level IL1B were tested with 20 replicates across one plate.
  Inter-assay Precision: 3 samples with known low, middle and high level IL1B were tested on three different plates with 8 replicates, respectively.
  Intra-Assay CV: <15%
  Inter-Assay CV: <15%
• ELISA Kit Component:
  • IL1B Microplate: 96 Wells (12 x 8 Well strips)
  • IL1B Lyophilized Standard: 2
  • 100X Biotinylated IL1B Detector Anitbody: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Sample Diluent: 1 x 50 mL
  • Detector Antibody Diluent: 1 x 15 mL
  • Conjugate Diluent: 1 x 15 mL
  • 25X Wash Buffer: 1 x 20 mL
  • Stop Solution: 1 x 10 mL
  • TMB Substrate: 1 x 10 mL
• Reconstitution and Storage: Store as indicated in product manual.
• Sample Type: Serum, Plasma, Tissue Homogenates
• Sensitivity: 0.78 pg/mL
• Assay Info: Assay Methodology: Quantitative Sandwich ELISA

Target Info

• Gene Symbol: IL1B
• Gene Full Name: Interleukin-1 beta
• Protein Name: Interleukin-1 beta
• Description of Target: Potent proinflammatory cytokine. Initially discovered as the major endogenous pyrogen, induces prostaglandin synthesis, neutrophil influx and activation, T-cell activation and cytokine production, B-cell activation and antibody production, and fibroblast proliferation and collagen production.
• Uniprot ID: P79162
• Protein Accession #: XP_013823154.1
• Nucleotide Accession #: XM_013967700.2