IL1B ELISA Kit (Bovine) (OKWB00115)

Catalog No: OKWB00115

Size:96T

Bovine IL1B ELISA Kit standard curve

Product Info

• Predicted Species Reactivity: Bos taurus|Bovine
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric, OD 450 nm
• ELISA Kit Duration: ~ 3 Hours
• ELISA Kit Linearity:

  Sample Type	1:2 Dilution	1:4 Dilution	1:8 Dilution	1:16 Dilution
  Serum (n=5)	86-105%	85-102%	86-103%	88-102%
  EDTA Plasma (n=5)	86-95%	86-100%	83-101%	89-97%
  Heparin Plasma (n=5)	80-99%	85-100%	88-100%	85-99%

• ELISA Kit Principle: Aviva Systems Biology IL1b ELISA Kit (Bovine) (OKWB00115) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for IL1b has been pre-coated onto a 96-well plate (12 x 8 Well Strips). Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for IL1b is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample IL1b captured in the well.
• ELISA Kit Range: 15.625-1000pg/ml
• ELISA Kit Recovery:

  Sample Type	Range	Mean
  Serum (n=5)	86-105	97
  EDTA Plasma (n=5)	89-101	94
  Heparin Plasma (n=5)	89-101	96

• ELISA Kit Reproducibility: Mean Intra-Assay Precision <10%
  Mean Inter-Assay Precision <12%
• ELISA Kit Component:
  • IL1b Microplate: 96 Wells (12 x 8 Well strips)
  • IL1b Lyophilized Standard: 2 x 5 ng
  • 100X IL1b Biotinylated Detector Anitbody: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Standard Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 25X Wash Buffer: 1 x 20 mL
  • TMB Substrate: 1 x 10 mL
  • Stop Solution: 1 x 10 mL
• Reconstitution and Storage: Store as indicated in product manual.
• Sample Type: Serum, Plasma, Tissue Homogenates & Other Biological Fluids
• Sensitivity: 46.9 pg/mL
• Assay Info: Assay Type: Quantitative Sandwich ELISA

Target Info

• Gene Symbol: IL1B
• Gene Full Name: interleukin 1 beta
• Alias Symbols: IL-1 beta;interleukin-1 beta.
• NCBI Gene Id: 281251
• Protein Name: Interleukin-1 beta
• Description of Target: Potent proinflammatory cytokine. Initially discovered as the major endogenous pyrogen, induces prostaglandin synthesis, neutrophil influx and activation, T-cell activation and cytokine production, B-cell activation and antibody production, and fibroblast proliferation and collagen production. Promotes Th17 differentiation of T-cells. Synergizes with IL12/interleukin-12 to induce IFNG synthesis from T-helper 1 (Th1) cells.
• Uniprot ID: P09428