IL1B Chemi-Luminescent ELISA Kit (Rat) (OKCD03636)

Catalog No: OKCD03636

Size:96WELLS

Rat IL1B ELISA Kit Standard Curve

Product Info

• Predicted Species Reactivity: Rat|Rattus norvegicus
• Application: Chemiluminescent immunoassay
• ELISA Kit Detection Method: Chemiluminescent
• ELISA Kit Duration: 2h, 40min
• ELISA Kit Linearity:

  Sample	1:2	1:4	1:8	1:16
  Serum (n=5)	92-101%	97-104%	81-95%	80-96%
  EDTA Plasma (n=5)	84-96%	81-101%	83-103%	84-96%
  Heparin Plasma (n=5)	90-98%	79-90%	83-93%	97-105%

• ELISA Kit Principle: The microplate provided in this kit has been pre-coated with an antibody specific to Interleukin 1 Beta (IL1b). Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to Interleukin 1 Beta (IL1b). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is proportional to the Interleukin 1 Beta (IL1b) level in the sample or standard.
• ELISA Kit Range: 1.37-1,000pg/mL
• ELISA Kit Recovery:

  Matrix	Recovery range (%)	Average(%)
  Serum (n=5)	87-103	91
  EDTA Plasma (n=5)	88-102	93
  Heparin Plasma (n=5)	90-104	101

• ELISA Kit Reproducibility: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Interleukin 1 Beta (IL1b) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Interleukin 1 Beta (IL1b) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
• ELISA Kit Component:
  • Il1b Microplate: 96 Wells (12 x 8 Well strips)
  • Il1b Lyophilized Standard: 2
  • 100X Il1b Biotinylated Detector Anitbody: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Standard Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 30X Wash Buffer: 1 x 20 mL
  • 100X Luminol Substrate: 1 x 2 mL
  • Substrate Diluent: 1 x 20 mL
• Reconstitution and Storage: 2°C to 8°C|-20°C
• Sample Type: Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
• Sensitivity: 1.37 pg/mL
• Assay Info: Assay Methodology: Quantitative Sandwich ELISA

Target Info

• Gene Symbol: Il1b
• Alias Symbols: IL-1F2
• NCBI Gene Id: 24494
• Protein Name: Interleukin-1 beta
• Description of Target: Potent proinflammatory cytokine. Initially discovered as the major endogenous pyrogen, induces prostaglandin synthesis, neutrophil influx and activation, T-cell activation and cytokine production, B-cell activation and antibody production, and fibroblast proliferation and collagen production. Promotes Th17 differentiation of T-cells. Synergizes with IL12/interleukin-12 to induce IFNG synthesis from T-helper 1 (Th1) cells.
• Uniprot ID: Q63264
• Protein Accession #: NP_113700
• Nucleotide Accession #: NM_031512