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IL1B Antibody (OASA07073)

0.5 mg
$589.00
In Stock
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Gene Symbol:
IL1B
NCBI Gene Id:
281251
Protein Name:
Interleukin-1 beta
Swissprot Id:
P09428
Protein Accession #:
NP_776518.1
Replacement Item:
This antibody may replace item sc-111184 from Santa Cruz Biotechnology.
Protein Size (# AA):
266
Host:
Rabbit
Clonality:
Polyclonal
Purification:
Antisera to bovine IL-1 beta were raised by repeated immunisation of rabbits with highly purified antigen. Purified IgG was prepared by affinity chromatography on protein G.
Application:
ELISA
Tissue Tool:
Find tissues and cell lines supported by DNA array analysis to express IL1B.
RNA Seq:
Find tissues and cell lines supported by RNA-seq analysis to express IL1B.
Immunogen:
The immunogen for anti-IL1B antibody: recombinant bovine IL-1 beta.
Predicted Species Reactivity:
Cow
Predicted Homology Based on Immunogen Sequence:
Cow
Product Format:
Purified IgG - liquid. Azide Free. Supplied in Phosphate buffered saline.
Reconstitution and Storage:
Store at -20C only. Ship in 4C
This product should be stored undiluted.
Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Datasheets/Manuals:
Printable datasheet for anti-IL1B antibody - OASA07073
Specificity:
Rabbit anti Bovine Interleukin-1 beta antibody recognizes bovine interleukin-1 beta (IL-1 beta). IL-1 beta is principally expressed by macrophages, and plays an important immunomodulatory role in the bovine immune response.
Rabbit anti Bovine Interleukin-1 beta antibody recognizes both native and recombinant bovine IL-1 beta. No detectable cross-reactivity was observed with human, mouse or rat IL-1 beta.
Isotype:
IgG
Application Info:
ELISA : Suggested dilutionL 1 ug/ml - 10 ug/ml. This antibody may be used in an indirect ELISA or as a capture antibody in a sandwich ELISA system, in combination with OASA07074 as the detection antibody. OPSA10927 may be used as a standard.
Additional Information:
Preservative Stabilisers: None present
:::
Approx Protein Conc: IgG concentration 1.0 mg/ml
Target Reference:
1. Wenz, J.R. et al. (2010) Factors associated with concentrations of select cytokine and acute phase proteins in dairy cows with naturally occurring clinical mastitis. J Dairy Sci. 93: 2458-70.
2. Rinaldi, M. et al (2010) A sentinel function for teat tissues in dairy cows: dominant innate immune response elements define early response to E. coli mastitis. Funct Integr Genomics. 10: 21-38.

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