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Catalog No: OKCD04248
Size:96WELLS
Price: $800.00
SKU
OKCD04248
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Datasheets/ManualsPrintable datasheet for IL1A ELISA Kit (Sheep) (OKCD04248)
Product Info
Predicted Species ReactivityOvis aries|Sheep
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric
ELISA Kit Duration3h
ELISA Kit Linearity
Sample1:21:41:81:16
Serum (n=5)97-104%91-98%91-99%84-97%
EDTA Plasma (n=5)79-93%84-102%85-93%89-101%
Heparin Plasma (n=5)88-97%91-101%81-95%85-95%
ELISA Kit PrincipleThe test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Interleukin 1 Alpha (IL1a). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Interleukin 1 Alpha (IL1a). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Interleukin 1 Alpha (IL1a), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Interleukin 1 Alpha (IL1a) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
ELISA Kit Range7.8-500pg/mL
ELISA Kit Recovery
MatrixRecovery range (%)Average (%)
Serum (n=5)93-10197
EDTA Plasma (n=5)85-9995
Heparin Plasma (n=5)80-10397
ELISA Kit ReproducibilityIntra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Interleukin 1 Alpha (IL1a) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Interleukin 1 Alpha (IL1a) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
ELISA Kit Component
ComponentAmount
Il1a Microplate96 Wells (12 x 8 Well strips)
Il1a Lyophilized Standard2
100X Il1a HRP-Detector Anitbody1 x 120 uL
100X Avidin-HRP Conjugate1 x 120 uL
Standard Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
30X Wash Buffer1 x 20 mL
100X Luminol Substrate1 x 2 mL
Substrate Diluent1 x 20 mL
Reconstitution and Storage2°C to 8°C|-20°C
Sample TypeSerum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.
Sensitivity6.0 pg/mL
Assay InfoAssay Methodology: Quantitative Sandwich ELISA
Gene SymbolIL1A
Gene Full Nameinterleukin 1 alpha
Alias SymbolsIL-1 alpha;interleukin-1 alpha;precursor interleukin 1 alpha.
NCBI Gene Id443404
Protein NameInterleukin-1 alpha
Description of TargetProduced by activated macrophages, IL-1 stimulates thymocyte proliferation by inducing IL-2 release, B-cell maturation and proliferation, and fibroblast growth factor activity. IL-1 proteins are involved in the inflammatory response, being identified as endogenous pyrogens, and are reported to stimulate the release of prostaglandin and collagenase from synovial cells.
Uniprot IDQ28579
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