Size:48 Wells
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IL1A ELISA Kit (Human) : 48 Wells (OKAA00007-48W)

Catalog#: OKAA00007-48W
Domestic: within 1-2 weeks delivery | International: 1-2 weeks
More Information
Predicted Species Reactivity Human
Application ELISA
Kit Detection Method Colorimetric, OD450 nm
Kit Reproducibility The coefficient of variation of both intra-assay and inter-assay were less than 10%.
Kit Component
2 vials human IL-1alpha Standard lyophilized, 500 pg/ml upon reconstitution
2 vials concentrated Biotin-Conjugate anti-human IL-1alpha monoclonal antibody
2 vials Streptavidin-HRP solution,
1 bottle Standard /sample Diluent
1 bottle Biotin-Conjugate antibody Diluent
1 bottle Streptavidin-HRP Diluent
1 bottle Wash Buffer Concentrate 20x (PBS with 1% Tween-20)
1 vial Substrate Solution
1 vial Stop Solution
Additional Information Sample Type: cell culture supernates, serum, and plasma
:: Reagents: REAGENTS
1. Aluminium pouches with a Microwell Plate coated with monoclonal antibody to human IL-1?(8x12)
2. 2 vials human IL-1? Standard lyophilized, 500 pg/ml upon reconstitution
3. 2 vials concentrated Biotin-Conjugate anti-human IL-1? monoclonal antibody
4. 2 vials Streptavidin-HRP solution,
5. 1 bottle Standard /sample Diluent
6. 1 bottle Biotin-Conjugate antibody Diluent
7. 1 bottle Streptavidin-HRP Diluent
8. 1 bottle Wash Buffer Concentrate 20x (PBS with 1% Tween-20)
9. 1 vial Substrate Solution
10. 1 vial Stop Solution
11. 3 pieces Adhesive Films
12. package insert
Sample Type cell culture, supernates, serum, and plasma
Sensitivity 4 pg/ml
Predicted Homology Based on Immunogen Sequence Human
Datasheets/Manuals Click here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Specificity This assay recognizes both natural and recombinant human IFN-gamma. The factors listed below were prepared at 50ng/ml in Standard /sample Diluent and assayed for cross-reactivity and no significant cross-reactivity or interference was observed.
Recombinant human: IL-6, IL-8, TNF-alpha, TNF-b, VEGF
Recombinant mouse: IL-1a, IFN-gamma
Recombinant porcine: IL-1b, IFN-gamma
Assay Info Assay Method: Quantitative Sandwich ELISA
Gene Symbol IL1A
Official Gene Full Name interleukin 1 alpha
Alias Symbols Hematopoietin-1, IL1, IL-1A, IL1-ALPHA, IL-1 alpha, IL1F1, Interleukin-1 alpha
NCBI Gene Id 3552
Protein Name Interleukin-1 alpha
Description of Target The protein encoded by this gene is a member of the interleukin 1 cytokine family. This cytokine is a pleiotropic cytokine involved in various immune responses, inflammatory processes, and hematopoiesis. This cytokine is produced by monocytes and macrophages as a proprotein, which is proteolytically processed and released in response to cell injury, and thus induces apoptosis. This gene and eight other interleukin 1 family genes form a cytokine gene cluster on chromosome 2. It has been suggested that the polymorphism of these genes is associated with rheumatoid arthritis and Alzheimer's disease.
Swissprot Id P01583
Protein Accession # NP_000566
Nucleotide Accession # NM_000575
Tissue Tool Find tissues and cell lines supported by DNA array analysis to express IL1A.
RNA Seq Find tissues and cell lines supported by RNA-seq analysis to express IL1A.
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