Catalog No: OKCD07550
Size:96WELLS
Price: $750.00
SKU
OKCD07550
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Datasheets/ManualsPrintable datasheet for IL18R1 ELISA Kit (Human) (OKCD07550)
Product Info
Predicted Species ReactivityHomo sapiens|Human
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric
ELISA Kit Duration3h
ELISA Kit PrincipleThe test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Interleukin 18 Receptor 1 (IL18R1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Interleukin 18 Receptor 1 (IL18R1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Interleukin 18 Receptor 1 (IL18R1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Interleukin 18 Receptor 1 (IL18R1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
ELISA Kit Range15.6-1,000pg/mL
ELISA Kit ReproducibilityIntra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Interleukin 18 Receptor 1 (IL18R1) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Interleukin 18 Receptor 1 (IL18R1) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
ELISA Kit Component
ComponentAmount
IL18R1 Microplate96 Wells (12 x 8 Well strips)
IL18R1 Lyophilized Standard2
100X IL18R1 HRP-Detector Anitbody1 x 120 uL
100X Avidin-HRP Conjugate1 x 120 uL
Standard Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
30X Wash Buffer1 x 20 mL
TMB Substrate1 x 2 mL
Stop Solution1 x 20 mL
Reconstitution and Storage2°C to 8°C|-20°C
Sample TypeSerum, plasma, tissue homogenates and other biological fluids.
Sensitivity< 0.058ng/mL
Gene SymbolIL18R1
Gene Full Nameinterleukin 18 receptor 1
Alias SymbolsCD218 antigen-like family member A;CD218a;CDw218a;cytokine receptor;IL1 receptor-related protein;IL18RA;IL-18Ralpha;IL-18R-alpha;IL18Ralpha2;IL-1Rrp;IL1RRP;interleukin-18 receptor 1;interleukin-18 receptor alpha.
NCBI Gene Id8809
Protein NameInterleukin-18 receptor 1
Description of TargetWithin the IL18 receptor complex, responsible for the binding of the proinflammatory cytokine IL18, but not IL1A nor IL1B (PubMed:8626725, PubMed:14528293, PubMed:25261253, PubMed:25500532). Involved in IL18-mediated IFNG synthesis from T-helper 1 (Th1) cells (PubMed:10653850). Contributes to IL18-induced cytokine production, either independently of SLC12A3, or as a complex with SLC12A3 (By similarity).
Uniprot IDQ13478
Protein Accession #NP_001269328.1
Nucleotide Accession #NM_001282399.1
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