Catalog No: OKCD03628
Size:96WELLS
Price: $837.00
SKU
OKCD03628
Availability: Domestic: within 1-2 weeks delivery | International: within 1-2 weeks delivery
Please contact customer service when ordering 10+ kits.
Contact Us:
- Toll Free: 888-880-0001
- Phone: 858-552-6979
- Email: info@avivasysbio.com
Shipping Info:
- $55: Antibody & Protein in US
- $55 + $25/Kit in US
- Contact us for international orders.
Datasheets/Manuals | Printable datasheet for IL18 Chemi-Luminescent ELISA Kit (Rat) (OKCD03628) |
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Predicted Species Reactivity | Rat|Rattus norvegicus | ||||||||||||||||||||||
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Application | Chemiluminescent immunoassay | ||||||||||||||||||||||
ELISA Kit Detection Method | Chemiluminescent | ||||||||||||||||||||||
ELISA Kit Duration | 2h, 40min | ||||||||||||||||||||||
ELISA Kit Linearity |
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ELISA Kit Principle | The microplate provided in this kit has been pre-coated with an antibody specific to Interleukin 18 (IL18). Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to Interleukin 18 (IL18). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is proportional to the Interleukin 18 (IL18) level in the sample or standard. | ||||||||||||||||||||||
ELISA Kit Range | 2.74-2,000pg/mL | ||||||||||||||||||||||
ELISA Kit Recovery |
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ELISA Kit Reproducibility | Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Interleukin 18 (IL18) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Interleukin 18 (IL18) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12% | ||||||||||||||||||||||
ELISA Kit Component |
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Reconstitution and Storage | 2°C to 8°C|-20°C | ||||||||||||||||||||||
Sample Type | Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids | ||||||||||||||||||||||
Sensitivity | 2.74 pg/mL | ||||||||||||||||||||||
Assay Info | Assay Methodology: Quantitative Sandwich ELISA |
Gene Symbol | Il18 |
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Gene Full Name | interleukin 18 |
Alias Symbols | IFN-gamma-inducing factor;IL-1 gamma;IL-18;interferon gamma-inducing factor;interleukin-1 gamma;interleukin-18. |
NCBI Gene Id | 29197 |
Protein Name | Interleukin-18 |
Description of Target | A proinflammatory cytokine primarily involved in polarized T-helper 1 (Th1) cell and natural killer (NK) cell immune responses. Upon binding to IL18R1 and IL18RAP, forms a signaling ternary complex which activates NF-kappa-B, triggering synthesis of inflammatory mediators. Synergizes with IL12/interleukin-12 to induce IFNG synthesis from T-helper 1 (Th1) cells and natural killer (NK) cells. |
Uniprot ID | P97636 |
Protein Accession # | NP_062038 |
Nucleotide Accession # | NM_019165 |
- Protocol:
- Reconstitution & Storage Instructions
- Western Blotting/Immunoblotting (WB/IB) Protocol
- Immunohistochemistry (IHC) Protocol
- Immunocytochemistry (ICC) Protocol
- Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
- Blocking Peptide Competition Protocol (BPCP)
- Immunoprecipitation (IP) Protocol
- Antibody Array (AA) Protocol
- Tips Information:
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See our General FAQ page.
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