IL17B ELISA Kit (Human) (OKCD07610)

Catalog No: OKCD07610

Size:96WELLS

IL17B ELISA Kit (Human) (OKCD07610) Standard Curve

Product Info

• Predicted Species Reactivity: Homo sapiens|Human
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric
• ELISA Kit Duration: 3h
• ELISA Kit Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Interleukin 17B (IL17B). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Interleukin 17B (IL17B). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Interleukin 17B (IL17B), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Interleukin 17B (IL17B) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
• ELISA Kit Range: 15.6-1,000pg/mL
• ELISA Kit Reproducibility: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Interleukin 17B (IL17B) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Interleukin 17B (IL17B) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
• ELISA Kit Component:
  • IL17B Microplate: 96 Wells (12 x 8 Well strips)
  • IL17B Lyophilized Standard: 2
  • 100X IL17B HRP-Detector Anitbody: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Standard Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 30X Wash Buffer: 1 x 20 mL
  • TMB Substrate: 1 x 2 mL
  • Stop Solution: 1 x 20 mL
• Reconstitution and Storage: 2°C to 8°C|-20°C
• Sample Type: Serum, plasma and other biological fluids.
• Sensitivity: < 5.6pg/mL

Target Info

• Gene Symbol: IL17B
• Gene Full Name: interleukin 17B
• Alias Symbols: Cytokine Zcyto7;cytokine-like protein ZCYTO7;IL-17B;IL-20;interleukin 20;interleukin-17 beta;interleukin-17B;Interleukin-20;neuronal interleukin-17 related factor;Neuronal interleukin-17-related factor;NIRF;ZCYTO7.
• NCBI Gene Id: 27190
• Protein Name: Interleukin-17B
• Description of Target: Stimulates the release of tumor necrosis factor alpha and IL-1-beta from the monocytic cell line THP-1.
• Uniprot ID: Q9UHF5
• Protein Accession #: NP_001304916.1
• Nucleotide Accession #: NM_001317987.1