IL17A ELISA Kit (Human) : 96 Wells (OKAA00018-96W)

Catalog No: OKAA00018-96W

Size:96 Wells

Standard curve of IL-17 protein using OKAA00018_96W

Product Info

• Predicted Species Reactivity: Human
• Application: ELISA
• ELISA Kit Detection Method: Colorimetric, OD450 nm
• ELISA Kit Reproducibility: The coefficient of variation of both intra-assay and inter-assay were less than 10%.
• ELISA Kit Component:

  Components
  2 vials human IL-17 Standard lyophilized, 1000 pg/ml upon reconstitution
  2 vials concentrated Biotin-Conjugate anti-human IL-17 antibody
  2 vials Streptavidin-HRP solution
  1 bottle Standard /sample Diluent
  1 bottle Biotin-Conjugate antibody Diluent
  1 bottle Streptavidin-HRP Diluent
  1 bottle Wash Buffer Concentrate 20x (PBS with 1% Tween-20)
  1 vial Substrate Solution
  1 vial Stop Solution
  2 vials human IL-21 Standard lyophilized, 1000 pg/ml upon reconstitution
  2 vials concentrated Biotin-Conjugate anti-human IL-21 antibody

• Additional Information: Sample Type: cell culture supernates, serum, and plasma
  Reagents: REAGENTS
  1. Aluminium pouches with a Microwell Plate coated with monoclonal antibody to human IL-17 (8x12)
  2. 2 vials human IL-17 Standard lyophilized, 1000 pg/ml upon reconstitution
  3. 2 vials concentrated Biotin-Conjugate anti-human IL-17 polyclonal antibody
  4. 2 vials Streptavidin-HRP solution,
  5. 1 bottle Standard /sample Diluent
  6. 1 bottle Biotin-Conjugate antibody Diluent
  7. 1 bottle Streptavidin-HRP Diluent
  8. 1 bottle Wash Buffer Concentrate 20x (PBS with 1% Tween-20)
  9. 1 vial Substrate Solution
  10. 1 vial Stop Solution
  11. 3 pieces Adhesive Films
  12. package insert
• Sample Type: cell culture, supernates, serum, and plasma
• Sensitivity: 7 pg/ml
• Predicted Homology Based on Immunogen Sequence: Human
• Specificity: This assay recognizes both natural and recombinant human IL-15. The factors listed below were prepared at 50ng/ml in Standard /sample Diluent and assayed for cross-reactivity and no significant cross-reactivity or interference was observed.
  Recombinant human: IL-1a, IL-1b, IL-2, IL-3, IL-4, IL-5, IL-6 ,
  Recombinant mouse: IL-1a, IL-1b, IL-2, IL-5, IL-6, IL-7 ,
  Other proteins: bovine FGF acidic, bovine FGF basic, human PDGF, porcine PDGF, human TGF-b1, porcine TGF-b1, bovine FGF acidic ,
• Assay Info: Assay Method: Quantitative Sandwich ELISA

Target Info

• Gene Symbol: IL17A
• Gene Full Name: interleukin 17A
• Alias Symbols: IL17, CTLA8, IL-17, CTLA-8, IL-17A
• NCBI Gene Id: 3605
• Protein Name: Interleukin-17A
• Description of Target: The protein encoded by this gene is a proinflammatory cytokine produced by activated T cells. This cytokine regulates the activities of NF-kappaB and mitogen-activated protein kinases. This cytokine can stimulate the expression of IL6 and cyclooxygenase-2 (PTGS2/COX-2), as well as enhance the production of nitric oxide (NO). High levels of this cytokine are associated with several chronic inflammatory diseases including rheumatoid arthritis, psoriasis and multiple sclerosis.
• Uniprot ID: Q16552
• Protein Accession #: NP_002181
• Nucleotide Accession #: NM_002190