Catalog No: OKCD03619
Size:96WELLS
Price: $793.00
SKU
OKCD03619
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IL17A Chemi-Luminescent ELISA Kit (Mouse) (OKCD03619)

Datasheets/ManualsPrintable datasheet for IL17A Chemi-Luminescent ELISA Kit (Mouse) (OKCD03619)
Product Info
Predicted Species ReactivityMouse|Mus musculus
ApplicationChemiluminescent immunoassay
ELISA Kit Detection MethodChemiluminescent
ELISA Kit Duration2h, 40min
ELISA Kit Linearity
Sample1:21:41:81:16
Serum (n=5)94-102%78-95%89-98%91-98%
EDTA Plasma (n=5)89-102%78-95%85-102%79-89%
Heparin Plasma (n=5)90-101%95-104%92-99%91-104%
ELISA Kit PrincipleThe microplate provided in this kit has been pre-coated with an antibody specific to Interleukin 17 (IL17). Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to Interleukin 17 (IL17). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is proportional to the Interleukin 17 (IL17) level in the sample or standard.
ELISA Kit Range2.74-2,000pg/mL
ELISA Kit Recovery
MatrixRecovery range (%)Average(%)
Serum (n=5)79-9895
EDTA Plasma (n=5)85-9793
Heparin Plasma (n=5)82-10598
ELISA Kit ReproducibilityIntra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Interleukin 17 (IL17) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Interleukin 17 (IL17) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
ELISA Kit Component
ComponentAmount
Il17a Microplate96 Wells (12 x 8 Well strips)
Il17a Lyophilized Standard2
100X Il17a Biotinylated Detector Anitbody1 x 120 uL
100X Avidin-HRP Conjugate1 x 120 uL
Standard Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
30X Wash Buffer1 x 20 mL
100X Luminol Substrate1 x 2 mL
Substrate Diluent1 x 20 mL
Reconstitution and Storage2°C to 8°C|-20°C
Sample TypeSerum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Sensitivity2.74 pg/mL
Assay InfoAssay Methodology: Quantitative Sandwich ELISA
Gene SymbolIl17a
Gene Full Nameinterleukin 17A
Alias SymbolsCtl;Ctla;Ctla8;Ctla-8;cytotoxic T-lymphocyte-associated antigen 8;Il;IL-;Il17;IL-17;IL-17A;interleukin-17A.
NCBI Gene Id16171
Protein NameInterleukin-17A
Description of TargetLigand for IL17RA (PubMed:17911633). The heterodimer formed by IL17A and IL17F is a ligand for the heterodimeric complex formed by IL17RA and IL17RC (By similarity). Involved in inducing stromal cells to produce proinflammatory and hematopoietic cytokines (By similarity).
Uniprot IDQ62386
Protein Accession #NP_034682
Nucleotide Accession #NM_010552
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