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Catalog No: OKEH00721 (Formerly GWB-3Z95NI)
Price: $525.00
Availability: Domestic: within 1-2 weeks delivery | International: 1-2 weeks
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Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Product Info
Predicted Species ReactivityHomo sapiens, Human
ELISA Kit Detection MethodColorimetric, OD450 nm
ELISA Kit Duration~ 3 Hours
ELISA Kit PrincipleAviva Systems Biology IL15RA ELISA Kit (Human) (OKEH00721) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for IL15RA has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for IL15RA is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample IL15RA captured in well.
ELISA Kit Range62.5-4000pg/mL
ELISA Kit RecoveryMean recovery when spiking into sample matrices at concentrations within the dynamic range: 99%
ELISA Kit ReproducibilityMean Intra-assay CV%: < 5.9% (n = 20)
Mean Inter-assay CV%: < 9.9% (n = 20)
ELISA Kit Component
IL15RA Microplate96 Wells (12 x 8 Well strips)
IL15RA Lyophilized Standard2
100X Biotinylated IL15RA Detector Antibody1 x 120 ÂuL
100X Avidin-HRP Conjugate1 x 120 ÂuL
Sample Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
25X Wash Buffer1 x 30 mL
TMB Substrate1 x 10 mL
Stop Solution1 x 10 mL
Reconstitution and StorageStore as indicated in product manual.
Sample TypeSerum, Plasma, Tissue homogenates, Cell culture supernates, Other biological fluids
Sensitivity15.6 pg/mL
SpecificityNatural and recombinant Human Interleukin-15 receptor subunit alpha
Assay InfoAssay Methodology: Quantitative Sandwich ELISA
Gene SymbolIL15RA
Gene Full Nameinterleukin 15 receptor subunit alpha
Alias SymbolsCD215;interleukin 15 receptor, alpha;interleukin-15 receptor subunit alpha.
NCBI Gene Id3601
Protein NameQ13261
Description of TargetInterleukin-15 receptor subunit alpha
Uniprot ID
Protein Accession #NP_001230468.1
Nucleotide Accession #NM_001243539.1
Protein Size (# AA)267
Molecular Weight28 kDa
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