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Catalog No: OKEH02969
Size:96T
Price: $425.00
SKU
OKEH02969
Availability: Domestic: within 1-2 weeks delivery | International: 1-2 weeks
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  • $55: Antibody & Protein in US
  • $55 + $25/Kit in US
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Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Please click here to view the MSDS.
Product Info
Predicted Species ReactivityRat, Rattus norvegicus
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric, OD450 nm
ELISA Kit Duration~ 3 Hours
ELISA Kit PrincipleAviva Systems Biology IL15 ELISA Kit (Rat) (OKEH02969) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Il15 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells and removed. A biotinylated detector antibody specific for Il15 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Il15 captured in well.
ELISA Kit Range15.6-1000pg/mL
ELISA Kit RecoveryMean recovery when spiking into Serum and Plasma = 105%
ELISA Kit ReproducibilityMean Intra-assay CV%: < 5.2% (n = 20)
Mean Inter-assay CV%: < 10.2% (n = 20)
ELISA Kit Component
ComponentAmount
Anti-Il15 Microplate96 Wells (12 x 8 Well strips)
Il15 Lyophilized Standard2
100X Biotinylated Il15 Detector Antibody1 x 120 uL
100X Avidin-HRP Conjugate1 x 120 uL
Sample Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
25X Wash Buffer1 x 30 mL
TMB Substrate1 x 10 mL
Stop Solution1 x 10 mL
Reconstitution and StorageStore as indicated in product manual.
Sample TypeSerum, Plasma, Urine, Tissue homogenates, Cell culture supernates, Other biological fluids
Sensitivity7.84 pg/mL
SpecificityNatural and recombinant Rat Interleukin-15
Assay InfoAssay Methodology: Quantitative Sandwich ELISA
Gene SymbolIl15
Gene Full Nameinterleukin 15
Alias SymbolsIL-15;interleukin-15.
NCBI Gene Id25670
Protein NameP97604
Description of TargetInterleukin-15
Uniprot IDhttps://www.uniprot.org/uniprot/P97604
Protein Accession #NP_037261.1
Nucleotide Accession #NM_013129.2
Protein Size (# AA)162
Molecular Weight19 kDa
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