IL15 ELISA Kit (Chicken) (OKWB00123)

Catalog No: OKWB00123

Size:96T

Chicken IL15 ELISA Kit standard curve

Product Info

• Predicted Species Reactivity: Chicken|Gallus gallus
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric, OD 450 nm
• ELISA Kit Duration: ~ 3 Hours
• ELISA Kit Linearity:

  Sample Type	1:2 Dilution	1:4 Dilution	1:8 Dilution	1:16 Dilution
  Serum (n=5)	87-102%	92-101%	86-105%	86-102%
  EDTA Plasma (n=5)	87-98%	82-101%	83-95%	82-99%
  Heparin Plasma (n=5)	87-97%	83-100%	82-95%	87-97%

• ELISA Kit Principle: Aviva Systems Biology IL15 ELISA Kit (Chicken) (OKWB00123) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for IL15 has been pre-coated onto a 96-well plate (12 x 8 Well Strips). Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for IL15 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample IL15 captured in the well.
• ELISA Kit Range: 15.625-1000pg/ml
• ELISA Kit Recovery:

  Sample Type	Range	Mean
  Serum (n=5)	86-104	95
  EDTA Plasma (n=5)	90-105	98
  Heparin Plasma (n=5)	85-97	91

• ELISA Kit Reproducibility: Mean Intra-Assay Precision <10%
  Mean Inter-Assay Precision <12%
• ELISA Kit Component:
  • IL15 Microplate: 96 Wells (12 x 8 Well strips)
  • IL15 Lyophilized Standard: 2 x 1 ng
  • 100X IL15 Biotinylated Detector Anitbody: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Standard Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 25X Wash Buffer: 1 x 20 mL
  • TMB Substrate: 1 x 10 mL
  • Stop Solution: 1 x 10 mL
• Reconstitution and Storage: Store as indicated in product manual.
• Sample Type: Serum, Plasma, Cell Cultures, Tissue Homogenates, Other Biological Fluids
• Sensitivity: 9.4 pg/mL
• Assay Info: Assay Type: Quantitative Sandwich ELISA

Target Info

• Gene Symbol: IL15
• Protein Name: Interleukin
• Uniprot ID: F1NZN5