IL13RA2 ELISA Kit (Human) (OKCD07544)

Catalog No: OKCD07544

Size:96WELLS

IL13RA2 ELISA Kit (Human) (OKCD07544) Standard Curve

Product Info

• Predicted Species Reactivity: Homo sapiens|Human
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric
• ELISA Kit Duration: 3h
• ELISA Kit Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Interleukin 13 Receptor Alpha 2 (IL13Ra2). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Interleukin 13 Receptor Alpha 2 (IL13Ra2). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Interleukin 13 Receptor Alpha 2 (IL13Ra2), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Interleukin 13 Receptor Alpha 2 (IL13Ra2) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
• ELISA Kit Range: 0.156-10ng/mL
• ELISA Kit Reproducibility: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Interleukin 13 Receptor Alpha 2 (IL13Ra2) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Interleukin 13 Receptor Alpha 2 (IL13Ra2) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
• ELISA Kit Component:
  • IL13RA2 Microplate: 96 Wells (12 x 8 Well strips)
  • IL13RA2 Lyophilized Standard: 2
  • 100X IL13RA2 HRP-Detector Anitbody: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Standard Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 30X Wash Buffer: 1 x 20 mL
  • TMB Substrate: 1 x 2 mL
  • Stop Solution: 1 x 20 mL
• Reconstitution and Storage: 2°C to 8°C|-20°C
• Sample Type: Serum, plasma, tissue homogenates, cell lysates and other biological fluids.
• Sensitivity: < 13.8pg/mL

Target Info

• Gene Symbol: IL13RA2
• Gene Full Name: interleukin 13 receptor subunit alpha 2
• Alias Symbols: cancer/testis antigen 19;CD213A2;CT19;IL-13 receptor subunit alpha-2;IL13BP;IL-13R;IL-13R subunit alpha-2;IL-13RA2;IL-13R-alpha-2;interleukin 13 binding protein;interleukin 13 receptor alpha 2 chain;interleukin 13 receptor, alpha 2;interleukin-13 receptor subunit alpha-2;Interleukin-13-binding protein.
• NCBI Gene Id: 3598
• Protein Name: Interleukin-13 receptor subunit alpha-2
• Description of Target: Binds as a monomer with high affinity to interleukin-13 (IL13), but not to interleukin-4 (IL4).
• Uniprot ID: Q14627
• Protein Accession #: NP_000631.1
• Nucleotide Accession #: NM_000640.2