Catalog No: OKCA01696
Size:96 Wells
Price: $754.00
SKU
OKCA01696
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Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Product Info
Predicted Species ReactivityMouse
ELISA Kit Detection MethodColorimetric, OD450 nm
ELISA Kit Duration~ 3.5 Hours
ELISA Kit Linearity
DilutionMean Recovery (%)Range (%)
Neat9687-100
1:210496-108
1:49283-97
1:8113102-117
ELISA Kit PrincipleAviva Systems Biology Il12rb2 ELISA Kit (Mouse) (OKCA01696) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Il12rb2 has been pre-coated onto a 96-well plate (12 x 8 Well Strips). Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for Il12rb2 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm and is quantitatively proportional to the amount of sample Il12rb2 captured in the well.
ELISA Kit Range0.156 - 10 ng/mL
ELISA Kit Recovery
SampleMean Recovery (%)Range (%)
Serum (n=5)9183-94
EDTA Plasma (n=4)10491-108
ELISA Kit Component
ComponentAmount
Il12rb2 Microplate96 Wells (12 x 8 Well strips)
Il12rb2 Lyophilized Standard2 x 10 ng
100X Biotinylated Il12rb2 Detector Anitbody1 x 120 uL
100X Avidin-HRP Conjugate1 x 120 uL
Sample Diluent1 x 50 mL
Detector Antibody Diluent1 x 15 mL
Conjugate Diluent1 x 15 mL
25X Wash Buffer1 x 20 mL
Stop Solution1 x 10 mL
TMB Substrate1 x 10 mL
Reconstitution and StorageStore as indicated in product manual.
Sample TypeSerum, Plasma, Tissue Homogenates, Cell Lysates
Sensitivity0.039 ng/mL
Assay InfoAssay Methodology: Quantitative Sanadwich ELISA
Protocol Information
Gene SymbolIL12RB2
Gene Full Nameinterleukin 12 receptor, beta 2
Alias SymbolsIf, Ifnm, IL-12, IL-12RB2, A930027I18Rik
NCBI Gene Id16162
Protein NameInterleukin-12 receptor subunit beta-2
Description of TargetReceptor for interleukin-12. This subunit is the signaling component coupling to the JAK2/STAT4 pathway. Promotes the proliferation of T-cells as well as NK cells. Induces the promotion of T-cells towards the Th1 phenotype by strongly enhancing IFN-gamma production. Can also activate STAT3.
Uniprot IDP97378
Protein Accession #NP_001298070.1
Nucleotide Accession #NM_001311141.1
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