Catalog No: OKCD00265 (Formerly GWB-KBBGL5)
Size:96WELLS
Price: $650.00
SKU
OKCD00265
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Datasheets/ManualsPrintable datasheet for IL12RB2 ELISA Kit (Human) (OKCD00265)
Product Info
Predicted Species ReactivityHomo sapiens|Human
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric
ELISA Kit Duration3h
ELISA Kit Linearity
Sample1:2 Dilution1:4 Dilution1:8 Dilution1:16 Dilution
serum(n=5)86-102%82-94%99-105%78-101%
EDTA plasma(n=5)79-93%94-105%78-102%80-102%
heparin plasma(n=5)78-101%88-95%79-90%98-105%
ELISA Kit PrincipleThe test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Interleukin 12 Receptor Beta 2 (IL12Rb2). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Interleukin 12 Receptor Beta 2 (IL12Rb2). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Interleukin 12 Receptor Beta 2 (IL12Rb2), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Interleukin 12 Receptor Beta 2 (IL12Rb2) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
ELISA Kit Range0.312-20ng/mL
ELISA Kit Recovery
MatrixRecovery Range (%)Average (%)
serum(n=5)88-9792
EDTA plasma(n=5)87-10196
heparin plasma(n=5)98-105101
ELISA Kit ReproducibilityIntra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Interleukin 12 Receptor Beta 2 (IL12Rb2) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Interleukin 12 Receptor Beta 2 (IL12Rb2) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
ELISA Kit Component
ComponentAmount
Anti-IL12RB2 Microplate96 Wells (12 x 8 Well Strips)
IL12RB2 Lyophilized Standard2 x 10 ng
100X Biotinylated IL12RB2 Detector Antibody120 uL
Avidin/HRP Conjugate120 uL
Standard Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
30X Wash Buffer1 x 20 mL
TMB Substrate1 x 9 mL
Stop Solution1 x 6 mL
Reconstitution and Storage2°C to 8°C|-20°C
Sample TypeSerum, plasma, tissue homogenates and other biological fluids.
Sensitivity< 0.053 ng/mL
SpecificityThis assay has high sensitivity and excellent specificity for detection of Interleukin 12 Receptor Beta 2 (IL12Rb2). No significant cross-reactivity or interference between Interleukin 12 Receptor Beta 2 (IL12Rb2) and analogues was observed.
Assay InfoAssay Methodology: Quantitative Sandwich Immunoassay
Gene SymbolIL12RB2
Gene Full Nameinterleukin 12 receptor subunit beta 2
Alias SymbolsIL-12 receptor subunit beta-2;IL-12R subunit beta-2;interleukin 12 receptor, beta 2;interleukin-12 receptor beta-2 chain;interleukin-12 receptor subunit beta-2.
NCBI Gene Id3595
Protein NameInterleukin-12 receptor subunit beta-2
Description of TargetReceptor for interleukin-12. This subunit is the signaling component coupling to the JAK2/STAT4 pathway. Promotes the proliferation of T-cells as well as NK cells. Induces the promotion of T-cells towards the Th1 phenotype by strongly enhancing IFN-gamma production.
Uniprot IDQ99665
Protein Accession #NP_001245143
Nucleotide Accession #NM_001258214.1
Protein Size (# AA)862
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