IL12B ELISA Kit (Rabbit) (OKWB00410)

Catalog No: OKWB00410

Size:96T

Rabbit IL12B ELISA Kit standard curve

Product Info

• Predicted Species Reactivity: Oryctolagus cuniculus|Rabbit
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric, OD 450 nm
• ELISA Kit Duration: ~ 3 Hours
• ELISA Kit Linearity:

  Sample Type	1:2 Dilution	1:4 Dilution	1:8 Dilution	1:16 Dilution
  Serum (n=5)	86-105%	89-96%	89-105%	85-99%
  EDTA Plasma (n=5)	88-96%	85-99%	87-98%	85-98%
  Heparin Plasma (n=5)	85-98%	80-97%	86-97%	85-92%

• ELISA Kit Principle: Aviva Systems Biology IL12b ELISA Kit (Rabbit) (OKWB00410) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for IL12b has been pre-coated onto a 96-well plate (12 x 8 Well Strips). Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for IL12b is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample IL12b captured in the well.
• ELISA Kit Range: 31.25-2000pg/ml
• ELISA Kit Recovery:

  Sample Type	Range	Mean
  Serum (n=5)	91-97	95
  EDTA Plasma (n=5)	88-103	96
  Heparin Plasma (n=5)	94-104	99

• ELISA Kit Reproducibility: Mean Intra-Assay Precision <10%
  Mean Inter-Assay Precision <12%
• ELISA Kit Component:
  • IL12b Microplate: 96 Wells (12 x 8 Well strips)
  • IL12b Lyophilized Standard: 2 x 2 ng
  • 100X IL12b Biotinylated Detector Anitbody: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Standard Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 25X Wash Buffer: 1 x 20 mL
  • TMB Substrate: 1 x 10 mL
  • Stop Solution: 1 x 10 mL
• Reconstitution and Storage: Store as indicated in product manual.
• Sample Type: Serum, Plasma, Cell Cultures, Tissue Homogenates, Other Biological Fluids
• Sensitivity: 18.75 pg/mL
• Assay Info: Assay Type: Quantitative Sandwich ELISA

Target Info

• Gene Symbol: IL12B
• Gene Full Name: interleukin 12B
• Alias Symbols: Cytotoxic lymphocyte maturation factor 40 kDa subunit;IL-12 subunit p40;interleukin-12 subunit beta.
• NCBI Gene Id: 100354852
• Protein Name: Interleukin-12 subunit beta
• Description of Target: Cytokine that can act as a growth factor for activated T and NK cells, enhance the lytic activity of NK/lymphokine-activated killer cells, and stimulate the production of IFN-gamma by resting PBMC.
• Uniprot ID: G1TBD7
• Protein Accession #: XP_002710393
• Nucleotide Accession #: XM_002710347