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Catalog No: OKCD00130 (Formerly GWB-KBBIT4)
Size:96WELLS
Price: $750.00
SKU
OKCD00130
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Datasheets/ManualsPrintable datasheet for IL12B ELISA Kit (Horse) (OKCD00130)
Product Info
Predicted Species ReactivityEquus caballus|Horse
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric
ELISA Kit Duration3h
ELISA Kit Linearity
Sample1:2 Dilution1:4 Dilution1:8 Dilution1:16 Dilution
serum(n=5)97-104%97-104%78-94%94-101%
EDTA plasma(n=5)83-102%96-105%83-101%85-99%
heparin plasma(n=5)99-105%80-98%93-101%93-105%
ELISA Kit PrincipleThe test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Interleukin 12B (IL12B). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Interleukin 12B (IL12B). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Interleukin 12B (IL12B), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Interleukin 12B (IL12B) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
ELISA Kit Range15.62-1000pg/mL
ELISA Kit Recovery
MatrixRecovery Range (%)Average (%)
serum(n=5)85-9589
EDTA plasma(n=5)88-9894
heparin plasma(n=5)90-9893
ELISA Kit ReproducibilityIntra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Interleukin 12B (IL12B) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Interleukin 12B (IL12B) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
ELISA Kit Component
ComponentAmount
Anti-IL12B Microplate96 Wells (12 x 8 Well Strips)
IL12B Lyophilized Standard2 x 1 ng
100X Biotinylated IL12B Detector Antibody120 uL
Avidin/HRP Conjugate120 uL
Standard Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
30X Wash Buffer1 x 20 mL
TMB Substrate1 x 9 mL
Stop Solution1 x 6 mL
Reconstitution and Storage2°C to 8°C|-20°C
Sample TypeSerum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.
Sensitivity< 6.2 pg/mL
SpecificityThis assay has high sensitivity and excellent specificity for detection of Interleukin 12B (IL12B). No significant cross-reactivity or interference between Interleukin 12B (IL12B) and analogues was observed.
Assay InfoAssay Methodology: Quantitative Sandwich Immunoassay
Gene SymbolIL12B
Gene Full Nameinterleukin 12B
Alias SymbolsCLMF p40;cytotoxic lymphocyte maturation factor 40 kDa subunit;IL-12 p40;IL-12 subunit p40;IL-12B;interleukin 12 p40 subunit;interleukin 12 subunit p40;interleukin 12B (natural killer cell stimulatory factor 2, cytotoxic lymphocyte maturation factor 2, p40);interleukin-12 subunit beta.
NCBI Gene Id100034222
Protein NameInterleukin-12 subunit beta
Description of TargetCytokine that can act as a growth factor for activated T and NK cells, enhance the lytic activity of NK/lymphokine-activated killer cells, and stimulate the production of IFN-gamma by resting PBMC.
Uniprot IDQ9XSQ5
Protein Accession #NP_001075985
Nucleotide Accession #NM_001082516.1
Protein Size (# AA)329
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