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Catalog No: OKEH00095
Size:96T
Price: $651.00
SKU
OKEH00095
Availability: Domestic: within 1-2 weeks delivery | International: 1-2 weeks
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IL12B ELISA Kit (Dog) : 96 Wells (OKEH00095)

Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Product Info
Predicted Species ReactivityCanis lupus, Dog
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric, OD450 nm
ELISA Kit Duration~ 3 Hours
ELISA Kit PrincipleAviva Systems Biology IL12B ELISA Kit (Dog) (OKEH00095) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for IL12B has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for IL12B is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample IL12B captured in well.
ELISA Kit Range78-5000pg/ml
ELISA Kit RecoveryMean recovery when spiking into sample matrices at concentrations within the dynamic range: 101%
ELISA Kit ReproducibilityMean Intra-assay CV%: < 4.6% (n = 20)
Mean Inter-assay CV%: < 7.2% (n = 20)
ELISA Kit Component
ComponentAmount
IL12B Microplate96 Wells (12 x 8 Well strips)
IL12B Lyophilized Standard2
100X Biotinylated IL12B Detector Antibody1 x 120 uL
100X Avidin-HRP Conjugate1 x 120 uL
Sample Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
25X Wash Buffer1 x 30 mL
TMB Substrate1 x 10 mL
Stop Solution1 x 10 mL
Reconstitution and StorageStore as indicated in product manual.
Sample TypeSerum, Plasma, Urine, Tissue homogenates, Cell culture supernates, Other biological fluids
Sensitivity31 pg/mL
SpecificityNatural and recombinant Dog Interleukin-12 subunit beta
Assay InfoAssay Methodology: Quantitative Sandwich ELISA
Gene SymbolIL12B
Gene Full Nameinterleukin 12B
Alias SymbolsCLMF p40;cytotoxic lymphocyte maturation factor 40 kDa subunit;IL-12 subunit p40;IL-12B;interleukin 12B (natural killer cell stimulatory factor 2, cytotoxic lymphocyte maturation factor 2, p40);interleukin-12 p40 subunit;interleukin-12 subunit beta.
NCBI Gene Id403976
Protein NameQ28268
Description of TargetInterleukin-12 subunit beta
Uniprot IDhttps://www.uniprot.org/uniprot/Q28268
Protein Accession #NP_001003292
Nucleotide Accession #NM_001003292
Protein Size (# AA)329
Molecular Weight37 kDa
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