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IL12B Antibody - Biotin Conjugated (OASA00348)

0.25 mg
$780.00
In Stock
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Gene Symbol:
IL12B
NCBI Gene Id:
281857
Protein Name:
Interleukin-12 subunit beta
Swissprot Id:
P46282
Protein Accession #:
NP_776781.1
Alias Symbols:
IL12p40
Replacement Item:
This antibody may replace item sc-1282 from Santa Cruz Biotechnology.
Description of Target:
MOUSE ANTI BOVINE INTERLEUKIN-12:Biotin
Protein Size (# AA):
327
Host:
Mouse
Clonality:
Monoclonal
Application:
ELISA, FC
Tissue Tool:
Find tissues and cell lines supported by DNA array analysis to express IL12B.
RNA Seq:
Find tissues and cell lines supported by RNA-seq analysis to express IL12B.
Immunogen:
The immunogen for anti-IL12B antibody: recombinant bovine IL-12
Predicted Species Reactivity:
Cow
Predicted Homology Based on Immunogen Sequence:
Cow
Product Format:
Purified IgG conjugated to Biotin - liquid
Reconstitution and Storage:
Store at +4oC or at -20oC if preferred.
This product should be stored undiluted.
Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Datasheets/Manuals:
Printable datasheet for anti-IL12B antibody - OASA00348
Specificity:
IL-12
Clone:
CC326
Conjugation:
Biotin
Isotype:
IgG2b
Application Info:
ELISA : This biotin conjugate may be used as detection reagent in a sandwich ELISA assay using OASA00349 as capture reagent.
Flow Cytometry: Use 10ul of the suggested working dilution to label 106 cells of 100ul.
Additional Information:
Fusion Partners: Spleen cells from immunised BALB/c mice were fused with cells of the mouse SP2/0 myeloma cell line.
:::
Preparation: Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Preservative Stabilisers: 0.09% - Sodium Azide
:::
Approx Protein Conc: IgG concentration 1.0 mg/ml
Buffer Solutions: Phosphate buffered saline pH7.4
Target Reference:
1. Hope, J. C. et al. (2002) Development of detection methods for ruminant interleukin (IL) - 12. J. Immunol. Methods. 266: 117 - 126.
2. Wenz, J.R. et al. (2010) Factors associated with concentrations of select cytokine and acute phase proteins in dairy cows with naturally occurring clinical mastitis. J Dairy Sci. 93: 2458-70.
3. Rinaldi, M. et al (2010) A sentinel function for teat tissues in dairy cows: dominant innate immune response elements define early response to E. coli mastitis. Funct Integr Genomics. 10: 21-38.

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