- NCBI Gene Id:
- Protein Name:
- Interleukin-12 subunit beta
- Alias Symbols:
- Replacement Item:
- This antibody may replace item sc-1282 from Santa Cruz Biotechnology.
- Description of Target:
- MOUSE ANTI BOVINE INTERLEUKIN-12:Biotin
- Protein Size (# AA):
- ELISA, FC
- Tissue Tool:
- Find tissues and cell lines supported by DNA array analysis to express IL12B.
- RNA Seq:
- Find tissues and cell lines supported by RNA-seq analysis to express IL12B.
- The immunogen for anti-IL12B antibody: recombinant bovine IL-12
- Predicted Species Reactivity:
- Predicted Homology Based on Immunogen Sequence:
- Product Format:
- Purified IgG conjugated to Biotin - liquid
- Reconstitution and Storage:
- Store at +4oC or at -20oC if preferred.
This product should be stored undiluted.
Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
- Printable datasheet for anti-IL12B antibody - OASA00348
- Application Info:
- ELISA : This biotin conjugate may be used as detection reagent in a sandwich ELISA assay using OASA00349 as capture reagent.
Flow Cytometry: Use 10ul of the suggested working dilution to label 106 cells of 100ul.
- Additional Information:
- Fusion Partners: Spleen cells from immunised BALB/c mice were fused with cells of the mouse SP2/0 myeloma cell line.
- Preparation: Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Preservative Stabilisers: 0.09% - Sodium Azide
- Approx Protein Conc: IgG concentration 1.0 mg/ml
Buffer Solutions: Phosphate buffered saline pH7.4
- Target Reference:
- 1. Hope, J. C. et al. (2002) Development of detection methods for ruminant interleukin (IL) - 12. J. Immunol. Methods. 266: 117 - 126.
2. Wenz, J.R. et al. (2010) Factors associated with concentrations of select cytokine and acute phase proteins in dairy cows with naturally occurring clinical mastitis. J Dairy Sci. 93: 2458-70.
3. Rinaldi, M. et al (2010) A sentinel function for teat tissues in dairy cows: dominant innate immune response elements define early response to E. coli mastitis. Funct Integr Genomics. 10: 21-38.
- Tips Information: