Catalog No: OKCD07903
Size:96WELLS
Price: $825.00
SKU
OKCD07903
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Datasheets/ManualsPrintable datasheet for IL12A ELISA Kit (Mouse) (OKCD07903)
Product Info
Predicted Species ReactivityMouse|Mus musculus
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric
ELISA Kit Duration3h
ELISA Kit PrincipleThe microplate provided in this kit has been pre-coated with a monoclonal antibody specific to IL12A. Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated monoclonal antibody specific to IL27B. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain IL35, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of IL35 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
ELISA Kit Range7.8-500pg/mL
ELISA Kit ReproducibilityIntra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Interleukin 35 (IL35) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Interleukin 35 (IL35) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
ELISA Kit Component
ComponentAmount
IL12A Microplate96 Wells (12 x 8 Well strips)
IL12A Lyophilized Standard2
100X IL12A HRP-Detector Anitbody1 x 120 uL
100X Avidin-HRP Conjugate1 x 120 uL
Standard Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
30X Wash Buffer1 x 20 mL
TMB Substrate1 x 9 mL
Stop Solution1 x 6 mL
Reconstitution and Storage2°C to 8°C|-20°C
Sample TypeSerum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Sensitivity< 3.3pg/mL
Gene SymbolIl12a
Gene Full Nameinterleukin 12a
Alias SymbolsCLMF p35;cytotoxic lymphocyte maturation factor 35 kDa subunit;IL-12;IL-12 p35 subunit;IL-12 subunit p35;Il-12a;IL-12p35;interleukin 12a p35 subunit;interleukin-12 p35 subunit;interleukin-12 subunit alpha;Ll12a;p3;p35.
NCBI Gene Id16159
Protein NameInterleukin-12 subunit alpha
Description of TargetCytokine that can act as a growth factor for activated T and NK cells, enhance the lytic activity of NK/lymphokine-activated Killer cells, and stimulate the production of IFN-gamma by resting PBMC.
Uniprot IDP43431
Protein Accession #NP_001152896.1
Nucleotide Accession #NM_001159424.2
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