IL12A ELISA Kit (Mouse) : 96 Wells (OKAA00039-96W)

Catalog No: OKAA00039-96W

Size:96 Wells

Standard curve of Il12a protein using OKAA00039_96W

Product Info

• Predicted Species Reactivity: Mouse
• Application: ELISA
• ELISA Kit Detection Method: Colorimetric, OD450 nm
• ELISA Kit Reproducibility: The coefficient of variation of both intra-assay and inter-assay were less than 10%.
• ELISA Kit Component:

  Components
  2 vials mouse IL-12p70 Standard lyophilized, 2000 pg/ml upon reconstitution
  2 vials concentrated Biotin-Conjugate anti-mouse IL-12p70 antibody
  2 vials Streptavidin-HRP solution
  1 bottle Standard /sample Diluent
  1 bottle Biotin-Conjugate antibody Diluent
  1 bottle Streptavidin-HRP Diluent
  1 bottle Wash Buffer Concentrate 20x (PBS with 1% Tween-20)
  1 vial Substrate Solution
  1 vial Stop Solution
  2 vials mouse IL-25/IL-17E Standard lyophilized, 1000 pg/ml upon reconstitution
  2 vials concentrated Biotin-Conjugate anti-mouse IL-25/IL-17E antibody

• Additional Information: Sample Type: cell culture supernates, serum, and plasma.
  Reagents: REAGENTS
  1. Aluminum pouches with a Microwell Plate coated with monoclonal antibody to mouse IL-12p70 (8x12)
  2. 2 vials mouse IL-12p70 Standard lyophilized, 2000 pg/ml upon reconstitution
  3. 2 vials concentrated Biotin-Conjugate anti-mouse IL-12p70 antibody
  4. 2 vials Streptavidin-HRP solution,
  5. 1 bottle Standard /sample Diluent
  6. 1 bottle Biotin-Conjugate antibody Diluent
  7. 1 bottle Streptavidin-HRP Diluent
  8. 1 bottle Wash Buffer Concentrate 20x (PBS with 1% Tween-20)
  9. 1 vial Substrate Solution
  10. 1 vial Stop Solution
  11. 3 pieces Adhesive Films
  12. package insert
• Sample Type: cell culture, supernates, serum, and plasma
• Sensitivity: 7 pg/ml
• Predicted Homology Based on Immunogen Sequence: Mouse
• Specificity: This assay recognizes both natural and recombinant mouse IL-12/IL-23p40. The factors listed below were prepared at 50ng/ml in Standard /sample Diluent and assayed for cross-reactivity and no significant cross-reactivity or interference was observed.
  Recombinant human: IL-12/IL-23p40, IL-12p70
  Recombinant mouse: IL-1a, IL-1b, IL-3, IL-5, IL-6
  :
• Assay Info: Assay Method: Quantitative Sandwich ELISA

Target Info

• Gene Symbol: IL12A
• Gene Full Name: interleukin 12a
• Alias Symbols: p3, p35, IL-12, Ll12a, Il-12a, IL-12p35
• NCBI Gene Id: 16159
• Protein Name: Interleukin-12 subunit alpha
• Uniprot ID: P43431
• Protein Accession #: NP_001152896.1
• Nucleotide Accession #: NM_001159424.2